Terpene biosynthesis in glandular trichomes of hop

Guodong Wang, Li Tian, Naveed Aziz, Pierre Broun, Xinbin Dai, Ji He, Andrew King, Patrick X. Zhao, Richard A. Dixon

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Hop (Humulus lupulus L. Cannabaceae) is an economically important crop for the brewing industry, where it is used to impart flavor and aroma to beer, and has also drawn attention in recent years due to its potential pharmaceutical applications. Essential oils (mono- and sesquiterpenes), bitter acids (prenylated polyketides), and prenylflavonoids are the primary phytochemical components that account for these traits, and all accumulate at high concentrations in glandular trichomes of hop cones. To understand the molecular basis for terpene accumulation in hop trichomes, a trichome cDNA library was constructed and 9,816 cleansed expressed sequence tag (EST) sequences were obtained from random sequencing of 16,152 cDNA clones. The ESTs were assembled into 3,619 unigenes (1,101 contigs and 2,518 singletons). Putative functions were assigned to the unigenes based on their homology to annotated sequences in the GenBank database. Two mono- and two sesquiterpene synthases identified from the EST collection were expressed in Escherichia coli. Hop MONOTERPENE SYNTHASE2 formed the linear monterpene myrcene from geranyl pyrophosphate, whereas hop SESQUITERPENE SYNTHASE1 (HlSTS1) formed both caryophyllene and humulene from farnesyl pyrophosphate. Together, these enzymes account for the production of the major terpene constituents of the hop trichomes. HlSTS2 formed the minor sesquiterpene constituent germacrene A, which was converted to β-elemene on chromatography at elevated temperature. We discuss potential functions for other genes expressed at high levels in developing hop trichomes.

Original languageEnglish (US)
Pages (from-to)1254-1266
Number of pages13
JournalPlant Physiology
Volume148
Issue number3
DOIs
StatePublished - Nov 2008

Fingerprint

Trichomes
Humulus
hops
Terpenes
terpenoids
trichomes
biosynthesis
Sesquiterpenes
Expressed Sequence Tags
sesquiterpenoids
unigenes
pyrophosphates
expressed sequence tags
Cannabaceae
bitter acids
Humulus lupulus
humulene
polyketides
seed cones
brewing industry

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Wang, G., Tian, L., Aziz, N., Broun, P., Dai, X., He, J., ... Dixon, R. A. (2008). Terpene biosynthesis in glandular trichomes of hop. Plant Physiology, 148(3), 1254-1266. https://doi.org/10.1104/pp.108.125187

Terpene biosynthesis in glandular trichomes of hop. / Wang, Guodong; Tian, Li; Aziz, Naveed; Broun, Pierre; Dai, Xinbin; He, Ji; King, Andrew; Zhao, Patrick X.; Dixon, Richard A.

In: Plant Physiology, Vol. 148, No. 3, 11.2008, p. 1254-1266.

Research output: Contribution to journalArticle

Wang, G, Tian, L, Aziz, N, Broun, P, Dai, X, He, J, King, A, Zhao, PX & Dixon, RA 2008, 'Terpene biosynthesis in glandular trichomes of hop', Plant Physiology, vol. 148, no. 3, pp. 1254-1266. https://doi.org/10.1104/pp.108.125187
Wang G, Tian L, Aziz N, Broun P, Dai X, He J et al. Terpene biosynthesis in glandular trichomes of hop. Plant Physiology. 2008 Nov;148(3):1254-1266. https://doi.org/10.1104/pp.108.125187
Wang, Guodong ; Tian, Li ; Aziz, Naveed ; Broun, Pierre ; Dai, Xinbin ; He, Ji ; King, Andrew ; Zhao, Patrick X. ; Dixon, Richard A. / Terpene biosynthesis in glandular trichomes of hop. In: Plant Physiology. 2008 ; Vol. 148, No. 3. pp. 1254-1266.
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