Tensin1 and a previously undocumented family member, tensin2, positively regulate cell migration

Huaiyang Chen, Ian C. Duncan, Hormozd Bozorgchami, Su Hao Lo

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Tensin is a focal adhesion molecule that binds to actin filaments and participates in signaling pathways. In this study, we have characterized a previously undocumented tensin family member, tensin2/KIAA 1075. Human tensin2 cDNA encodes a 1,285-aa sequence that shares extensive homology with tensin1 at its amino- and carboxyl-terminal ends, which include the actin-binding domain, the Src homology 2 (SH2) domain, and the phosphotyrosine binding (PTB) domain. Analysis of the genomic structures of tensin1 and tensin2 further confirmed that they represent a single gene family. Examination of tensin2 mRNA distribution revealed that heart, kidney, skeletal muscle, and liver were tissues of high expression. The endogenous and recombinant tensin2 were expressed as a 170-kDa protein in NIH 3T3 cells. The subcellular localization of tensin2 was determined by transfection of green fluorescence protein (GFP)-tensin2 fusion construct. The results indicated that tensin2 is also localized to focal adhesions. Finally, functional analysis of tensin genes has demonstrated that expression of tensin genes is able to promote cell migration on fibronectin, indicating that the tensin family plays a role in regulating cell motility.

Original languageEnglish (US)
Pages (from-to)733-738
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number2
DOIs
StatePublished - Jan 22 2002

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Cell Movement
Focal Adhesions
NIH 3T3 Cells
Phosphotyrosine
src Homology Domains
Actin Cytoskeleton
Fibronectins
Genes
Transfection
Actins
Skeletal Muscle
Proteins
Complementary DNA
Fluorescence
Tensins
Kidney
Gene Expression
Messenger RNA
Liver

Keywords

  • Cell motility
  • Focal adhesion
  • KIAA 1075

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Tensin1 and a previously undocumented family member, tensin2, positively regulate cell migration. / Chen, Huaiyang; Duncan, Ian C.; Bozorgchami, Hormozd; Lo, Su Hao.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 2, 22.01.2002, p. 733-738.

Research output: Contribution to journalArticle

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