Abstract
Tensin is a focal adhesion molecule that is known to regulate cell adhesion, migration, and proliferation. Although there are four tensin homologs (TNS1, TNS2, TNS3, and CTEN/TNS4) in mammals, only one tensin gene is found in Caenorhabditis elegans. Sequence analysis suggests that Caenorhabditis elegans tensin is slightly closer aligned with human TNS1 than with other human tensins. To establish the role of TNS1 in Caenorhabditis elegans, we have generated TNS1 knockout (KO) worms by CRISPR-Cas9 and homologous recombination directed repair approaches. Lack of TNS1 does not appear to affect the development or gross morphology of the worms. Nonetheless, defecation cycles are significantly longer in TNS1 KO worms. In addition, their pharyngeal pumping rate is markedly faster, which is likely due to a shorter pump duration in the KO worms. These findings indicate that TNS1 is not required for the development and survival of Caenorhabditis elegans but point to a critical role in modulating defecation and pharyngeal pumping rates.
| Original language | English (US) |
|---|---|
| Journal | Biochemical and Biophysical Research Communications |
| DOIs | |
| State | Accepted/In press - Jan 1 2019 |
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Keywords
- Caenorhabditis elegans
- Defecation
- Focal adhesion
- Pharyngeal pumping
- Tensin
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Tensin regulates pharyngeal pumping in Caenorhabditis elegans. / Bruns, Aaron N.; Lo, Su Hao.
In: Biochemical and Biophysical Research Communications, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tensin regulates pharyngeal pumping in Caenorhabditis elegans
AU - Bruns, Aaron N.
AU - Lo, Su Hao
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Tensin is a focal adhesion molecule that is known to regulate cell adhesion, migration, and proliferation. Although there are four tensin homologs (TNS1, TNS2, TNS3, and CTEN/TNS4) in mammals, only one tensin gene is found in Caenorhabditis elegans. Sequence analysis suggests that Caenorhabditis elegans tensin is slightly closer aligned with human TNS1 than with other human tensins. To establish the role of TNS1 in Caenorhabditis elegans, we have generated TNS1 knockout (KO) worms by CRISPR-Cas9 and homologous recombination directed repair approaches. Lack of TNS1 does not appear to affect the development or gross morphology of the worms. Nonetheless, defecation cycles are significantly longer in TNS1 KO worms. In addition, their pharyngeal pumping rate is markedly faster, which is likely due to a shorter pump duration in the KO worms. These findings indicate that TNS1 is not required for the development and survival of Caenorhabditis elegans but point to a critical role in modulating defecation and pharyngeal pumping rates.
AB - Tensin is a focal adhesion molecule that is known to regulate cell adhesion, migration, and proliferation. Although there are four tensin homologs (TNS1, TNS2, TNS3, and CTEN/TNS4) in mammals, only one tensin gene is found in Caenorhabditis elegans. Sequence analysis suggests that Caenorhabditis elegans tensin is slightly closer aligned with human TNS1 than with other human tensins. To establish the role of TNS1 in Caenorhabditis elegans, we have generated TNS1 knockout (KO) worms by CRISPR-Cas9 and homologous recombination directed repair approaches. Lack of TNS1 does not appear to affect the development or gross morphology of the worms. Nonetheless, defecation cycles are significantly longer in TNS1 KO worms. In addition, their pharyngeal pumping rate is markedly faster, which is likely due to a shorter pump duration in the KO worms. These findings indicate that TNS1 is not required for the development and survival of Caenorhabditis elegans but point to a critical role in modulating defecation and pharyngeal pumping rates.
KW - Caenorhabditis elegans
KW - Defecation
KW - Focal adhesion
KW - Pharyngeal pumping
KW - Tensin
UR - http://www.scopus.com/inward/record.url?scp=85075832696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075832696&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2019.11.153
DO - 10.1016/j.bbrc.2019.11.153
M3 - Article
C2 - 31784086
AN - SCOPUS:85075832696
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
ER -