Tensile properties, collagen content, and crosslinks in connective tissues of the immature knee joint

Sriram V. Eleswarapu, Donald J. Responte, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Background: The major connective tissues of the knee joint act in concert during locomotion to provide joint stability, smooth articulation, shock absorption, and distribution of mechanical stresses. These functions are largely conferred by the intrinsic material properties of the tissues, which are in turn determined by biochemical composition. A thorough understanding of the structure-function relationships of the connective tissues of the knee joint is needed to provide design parameters for efforts in tissue engineering. Methodology/Principal Findings: The objective of this study was to perform a comprehensive characterization of the tensile properties, collagen content, and pyridinoline crosslink abundance of condylar cartilage, patellar cartilage, medial and lateral menisci, cranial and caudal cruciate ligaments (analogous to anterior and posterior cruciate ligaments in humans, respectively), medial and lateral collateral ligaments, and patellar ligament from immature bovine calves. Tensile stiffness and strength were greatest in the menisci and patellar ligament, and lowest in the hyaline cartilages and cruciate ligaments; these tensile results reflected trends in collagen content. Pyridinoline crosslinks were found in every tissue despite the relative immaturity of the joints, and significant differences were observed among tissues. Notably, for the cruciate ligaments and patellar ligament, crosslink density appeared more important in determining tensile stiffness than collagen content. Conclusions/Significance: To our knowledge, this study is the first to examine tensile properties, collagen content, and pyridinoline crosslink abundance in a direct head-to-head comparison among all of the major connective tissues of the knee. This is also the first study to report results for pyridinoline crosslink density that suggest its preferential role over collagen in determining tensile stiffness for certain tissues.

Original languageEnglish (US)
Article numbere26178
JournalPLoS One
Volume6
Issue number10
DOIs
StatePublished - Oct 13 2011

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Ligaments
knees
ligaments
Knee Joint
Tensile properties
joints (animal)
connective tissues
Connective Tissue
collagen
Collagen
Patellar Ligament
immatures
Tissue
Tibial Meniscus
cartilage
Anterior Cruciate Ligament
Cartilage
Joints
posterior cruciate ligament
Ankle Lateral Ligament

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tensile properties, collagen content, and crosslinks in connective tissues of the immature knee joint. / Eleswarapu, Sriram V.; Responte, Donald J.; Athanasiou, Kyriacos A.

In: PLoS One, Vol. 6, No. 10, e26178, 13.10.2011.

Research output: Contribution to journalArticle

Eleswarapu, Sriram V. ; Responte, Donald J. ; Athanasiou, Kyriacos A. / Tensile properties, collagen content, and crosslinks in connective tissues of the immature knee joint. In: PLoS One. 2011 ; Vol. 6, No. 10.
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