Teneurin-2 is expressed in tissues that regulate limb and somite pattern formation and is induced in vitro and in situ by FGF8

Richard P Tucker, Ruth Chiquet-Ehrismann, Marie Pierre Chevron, Doris Martin, Ronelle J. Hall, Beatrix P. Rubin

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Teneurin-2 is a member of a novel family of transmembrane proteins characterized to date in fish, birds, mammals, and Drosophila (e.g., the pair-rule gene product Ten-m). We have shown that teneurin-2 is expressed by neurons in the developing avian visual system in a pattern complementary to the expression of teneurin-1 and that recombinant teneurin-2 induces morphologic changes in neuronal cells in culture (Rubin et al., 1999). Here we have used cRNA probes to two newly identified splice variants and a teneurin-2-specific antibody to determine whether teneurin-2 is also expressed outside the nervous system. Both reverse transcriptase-polymerase chain reaction and in situ hybridization indicate that the three splice variants known so far are coexpressed at sites of pattern formation during development. Teneurin-2 mRNAs and protein are found in the developing limbs, somites, and craniofacial mesenchyme. In addition to expression of teneurin-2 by the apical ectodermal ridge, teneurin-2 transcripts also appear transiently at sites of tendon development. Teneurin-2 expression patterns were strikingly similar to those of fibroblast growth factor 8 (FGF8). In agreement with the overlapping expression pattern, FGF8-coated beads implanted into chicken limb buds induced the ectopic expression of teneurin-2 and soluble FGF8 induced teneurin-2 in limb explant cultures. Thus, teneurin-2 could act downstream of FGF8 during morphogenesis.

Original languageEnglish (US)
Pages (from-to)27-39
Number of pages13
JournalDevelopmental Dynamics
Volume220
Issue number1
DOIs
StatePublished - 2001

Keywords

  • Apical ectodermal ridge (AER)
  • DOC4
  • Dorsomedial lip
  • FGF8
  • Limb development
  • Neurestin
  • Odz
  • Somite morphogenesis
  • Ten-a
  • Ten-m

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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