Teneurin-2 is expressed in tissues that regulate limb and somite pattern formation and is induced in vitro and in situ by FGF8

Richard P Tucker, Ruth Chiquet-Ehrismann, Marie Pierre Chevron, Doris Martin, Ronelle J. Hall, Beatrix P. Rubin

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Teneurin-2 is a member of a novel family of transmembrane proteins characterized to date in fish, birds, mammals, and Drosophila (e.g., the pair-rule gene product Ten-m). We have shown that teneurin-2 is expressed by neurons in the developing avian visual system in a pattern complementary to the expression of teneurin-1 and that recombinant teneurin-2 induces morphologic changes in neuronal cells in culture (Rubin et al., 1999). Here we have used cRNA probes to two newly identified splice variants and a teneurin-2-specific antibody to determine whether teneurin-2 is also expressed outside the nervous system. Both reverse transcriptase-polymerase chain reaction and in situ hybridization indicate that the three splice variants known so far are coexpressed at sites of pattern formation during development. Teneurin-2 mRNAs and protein are found in the developing limbs, somites, and craniofacial mesenchyme. In addition to expression of teneurin-2 by the apical ectodermal ridge, teneurin-2 transcripts also appear transiently at sites of tendon development. Teneurin-2 expression patterns were strikingly similar to those of fibroblast growth factor 8 (FGF8). In agreement with the overlapping expression pattern, FGF8-coated beads implanted into chicken limb buds induced the ectopic expression of teneurin-2 and soluble FGF8 induced teneurin-2 in limb explant cultures. Thus, teneurin-2 could act downstream of FGF8 during morphogenesis.

Original languageEnglish (US)
Pages (from-to)27-39
Number of pages13
JournalDevelopmental Dynamics
Volume220
Issue number1
DOIs
StatePublished - 2001

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Fibroblast Growth Factor 8
Somites
Extremities
Limb Buds
Complementary RNA
Mesoderm
Reverse Transcriptase Polymerase Chain Reaction
Morphogenesis
Tendons
Nervous System
Drosophila
Birds
In Situ Hybridization
Mammals
Chickens
Fishes
Proteins
Cell Culture Techniques
Neurons
Messenger RNA

Keywords

  • Apical ectodermal ridge (AER)
  • DOC4
  • Dorsomedial lip
  • FGF8
  • Limb development
  • Neurestin
  • Odz
  • Somite morphogenesis
  • Ten-a
  • Ten-m

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Teneurin-2 is expressed in tissues that regulate limb and somite pattern formation and is induced in vitro and in situ by FGF8. / Tucker, Richard P; Chiquet-Ehrismann, Ruth; Chevron, Marie Pierre; Martin, Doris; Hall, Ronelle J.; Rubin, Beatrix P.

In: Developmental Dynamics, Vol. 220, No. 1, 2001, p. 27-39.

Research output: Contribution to journalArticle

Tucker, Richard P ; Chiquet-Ehrismann, Ruth ; Chevron, Marie Pierre ; Martin, Doris ; Hall, Ronelle J. ; Rubin, Beatrix P. / Teneurin-2 is expressed in tissues that regulate limb and somite pattern formation and is induced in vitro and in situ by FGF8. In: Developmental Dynamics. 2001 ; Vol. 220, No. 1. pp. 27-39.
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