Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo

Daniela Kenzelmann, Ruth Chiquet-Ehrismann, Nathaniel T. Leachman, Richard P Tucker

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Background. Teneurins are a unique family of transmembrane proteins conserved from C. elegans and D. melanogaster to mammals. In vertebrates there are four paralogs (teneurin-1 to -4), all of which are expressed prominently in the developing central nervous system. Results. Analysis of teneurin-1 expression in the developing chick brain by in situ hybridization and immunohistochemistry defined a unique, distinct expression pattern in interconnected regions of the brain. Moreover we found complementary patterns of teneurin-1 and-2 expression in many parts of the brain, including the retina, optic tectum, olfactory bulb, and cerebellum as well as in brain nuclei involved in processing of sensory information. Based on these expression patterns, we suspect a role for teneurins in neuronal connectivity. In contrast to the cell-surface staining of the antibody against the extracellular domain, an antibody recognizing the intracellular domain revealed nuclear staining in subpopulations of neurons and in undifferentiated mesenchyme. Western blot analysis of brain lysates showed the presence of N-terminal fragments of teneurin-1 containing the intracellular domain indicating that proteolytic processing occurs. Finally, the teneurin-1 intracellular domain was found to contain a nuclear localization signal, which is required for nuclear localization in transfected cells. Conclusion. Teneurin-1 and -2 are expressed by distinct interconnected populations of neurons in the developing central nervous system. Our data support the hypothesis that teneurins can be proteolytically processed leading to the release of the intracellular domain and its translocation to the nucleus.

Original languageEnglish (US)
Article number30
JournalBMC Developmental Biology
Volume8
DOIs
StatePublished - 2008

Fingerprint

Brain
Central Nervous System
Caenorhabditis elegans Proteins
Staining and Labeling
Neurons
Nuclear Localization Signals
Superior Colliculi
Antibodies
Olfactory Bulb
Mesoderm
Automatic Data Processing
Cerebellum
In Situ Hybridization
Vertebrates
Retina
teneurin-1
Mammals
Western Blotting
Immunohistochemistry
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo. / Kenzelmann, Daniela; Chiquet-Ehrismann, Ruth; Leachman, Nathaniel T.; Tucker, Richard P.

In: BMC Developmental Biology, Vol. 8, 30, 2008.

Research output: Contribution to journalArticle

Kenzelmann, Daniela ; Chiquet-Ehrismann, Ruth ; Leachman, Nathaniel T. ; Tucker, Richard P. / Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo. In: BMC Developmental Biology. 2008 ; Vol. 8.
@article{93f325839afa45db9d38eec36931d1ce,
title = "Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo",
abstract = "Background. Teneurins are a unique family of transmembrane proteins conserved from C. elegans and D. melanogaster to mammals. In vertebrates there are four paralogs (teneurin-1 to -4), all of which are expressed prominently in the developing central nervous system. Results. Analysis of teneurin-1 expression in the developing chick brain by in situ hybridization and immunohistochemistry defined a unique, distinct expression pattern in interconnected regions of the brain. Moreover we found complementary patterns of teneurin-1 and-2 expression in many parts of the brain, including the retina, optic tectum, olfactory bulb, and cerebellum as well as in brain nuclei involved in processing of sensory information. Based on these expression patterns, we suspect a role for teneurins in neuronal connectivity. In contrast to the cell-surface staining of the antibody against the extracellular domain, an antibody recognizing the intracellular domain revealed nuclear staining in subpopulations of neurons and in undifferentiated mesenchyme. Western blot analysis of brain lysates showed the presence of N-terminal fragments of teneurin-1 containing the intracellular domain indicating that proteolytic processing occurs. Finally, the teneurin-1 intracellular domain was found to contain a nuclear localization signal, which is required for nuclear localization in transfected cells. Conclusion. Teneurin-1 and -2 are expressed by distinct interconnected populations of neurons in the developing central nervous system. Our data support the hypothesis that teneurins can be proteolytically processed leading to the release of the intracellular domain and its translocation to the nucleus.",
author = "Daniela Kenzelmann and Ruth Chiquet-Ehrismann and Leachman, {Nathaniel T.} and Tucker, {Richard P}",
year = "2008",
doi = "10.1186/1471-213X-8-30",
language = "English (US)",
volume = "8",
journal = "BMC Developmental Biology",
issn = "1471-213X",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo

AU - Kenzelmann, Daniela

AU - Chiquet-Ehrismann, Ruth

AU - Leachman, Nathaniel T.

AU - Tucker, Richard P

PY - 2008

Y1 - 2008

N2 - Background. Teneurins are a unique family of transmembrane proteins conserved from C. elegans and D. melanogaster to mammals. In vertebrates there are four paralogs (teneurin-1 to -4), all of which are expressed prominently in the developing central nervous system. Results. Analysis of teneurin-1 expression in the developing chick brain by in situ hybridization and immunohistochemistry defined a unique, distinct expression pattern in interconnected regions of the brain. Moreover we found complementary patterns of teneurin-1 and-2 expression in many parts of the brain, including the retina, optic tectum, olfactory bulb, and cerebellum as well as in brain nuclei involved in processing of sensory information. Based on these expression patterns, we suspect a role for teneurins in neuronal connectivity. In contrast to the cell-surface staining of the antibody against the extracellular domain, an antibody recognizing the intracellular domain revealed nuclear staining in subpopulations of neurons and in undifferentiated mesenchyme. Western blot analysis of brain lysates showed the presence of N-terminal fragments of teneurin-1 containing the intracellular domain indicating that proteolytic processing occurs. Finally, the teneurin-1 intracellular domain was found to contain a nuclear localization signal, which is required for nuclear localization in transfected cells. Conclusion. Teneurin-1 and -2 are expressed by distinct interconnected populations of neurons in the developing central nervous system. Our data support the hypothesis that teneurins can be proteolytically processed leading to the release of the intracellular domain and its translocation to the nucleus.

AB - Background. Teneurins are a unique family of transmembrane proteins conserved from C. elegans and D. melanogaster to mammals. In vertebrates there are four paralogs (teneurin-1 to -4), all of which are expressed prominently in the developing central nervous system. Results. Analysis of teneurin-1 expression in the developing chick brain by in situ hybridization and immunohistochemistry defined a unique, distinct expression pattern in interconnected regions of the brain. Moreover we found complementary patterns of teneurin-1 and-2 expression in many parts of the brain, including the retina, optic tectum, olfactory bulb, and cerebellum as well as in brain nuclei involved in processing of sensory information. Based on these expression patterns, we suspect a role for teneurins in neuronal connectivity. In contrast to the cell-surface staining of the antibody against the extracellular domain, an antibody recognizing the intracellular domain revealed nuclear staining in subpopulations of neurons and in undifferentiated mesenchyme. Western blot analysis of brain lysates showed the presence of N-terminal fragments of teneurin-1 containing the intracellular domain indicating that proteolytic processing occurs. Finally, the teneurin-1 intracellular domain was found to contain a nuclear localization signal, which is required for nuclear localization in transfected cells. Conclusion. Teneurin-1 and -2 are expressed by distinct interconnected populations of neurons in the developing central nervous system. Our data support the hypothesis that teneurins can be proteolytically processed leading to the release of the intracellular domain and its translocation to the nucleus.

UR - http://www.scopus.com/inward/record.url?scp=41849103389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41849103389&partnerID=8YFLogxK

U2 - 10.1186/1471-213X-8-30

DO - 10.1186/1471-213X-8-30

M3 - Article

C2 - 18366734

AN - SCOPUS:41849103389

VL - 8

JO - BMC Developmental Biology

JF - BMC Developmental Biology

SN - 1471-213X

M1 - 30

ER -