Tenascin-Y is concentrated in adult nerve roots and has barrier properties in vitro

Richard P Tucker, Carmen Hagios, Alicia Santiago, Ruth Chiquet-Ehrismann

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Several molecules have been identified as potential sources of the barriers to glial cell mixing and sensory regeneration that exist at the boundary between the peripheral and central nervous systems, including tenascin-C, tenascin-R, chondroitin sulfate proteoglycans, and NG2. Here we show that tenascin-Y, the avian homologue of tenascin-X, is concentrated in the proximal portions of peripheral nerves in the chicken. In vitro analyses of cultures enriched for Schwann cells demonstrate that recombinant tenascin-Y has dose-dependent effects on glial cell attachment, spreading, and migration. In addition, nanomolar concentrations of tenascin-Y cause the rapid collapse of sensory growth cones cultured on fibronectin, and regenerating sensory neurites preferentially migrate on fibronectin and avoid tenascin-Y in microstripe assays. We conclude that the expression pattern of tenascin-Y and its properties in vitro are consistent with a role as an inhibitor of glial cell migration and sensory regeneration in nerve roots.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalJournal of Neuroscience Research
Volume66
Issue number3
DOIs
StatePublished - Nov 1 2001

Fingerprint

Tenascin
Neuroglia
Fibronectins
Chondroitin Sulfate Proteoglycans
Growth Cones
Nerve Regeneration
Schwann Cells
Peripheral Nervous System
Neurites
Peripheral Nerves
Cell Movement
In Vitro Techniques
Regeneration
Chickens
Central Nervous System

Keywords

  • Chicken
  • Dorsal root entry zone
  • Dorsal rootlet
  • Extracellular matrix
  • Fibronectin
  • Microstripe assay
  • Regeneration
  • Schwann cell
  • Sensory neuron

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tenascin-Y is concentrated in adult nerve roots and has barrier properties in vitro. / Tucker, Richard P; Hagios, Carmen; Santiago, Alicia; Chiquet-Ehrismann, Ruth.

In: Journal of Neuroscience Research, Vol. 66, No. 3, 01.11.2001, p. 439-447.

Research output: Contribution to journalArticle

Tucker, Richard P ; Hagios, Carmen ; Santiago, Alicia ; Chiquet-Ehrismann, Ruth. / Tenascin-Y is concentrated in adult nerve roots and has barrier properties in vitro. In: Journal of Neuroscience Research. 2001 ; Vol. 66, No. 3. pp. 439-447.
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