Tenascin-W Is a Novel Stromal Marker in Biliary Tract Cancers

Ismaïl Hendaoui, Ahlem Lahmar, Luca Campo, Sihem Mebarki, Sandrine Bichet, Daniel Hess, Martin Degen, Nidhameddine Kchir, Leila Charrada-Ben Farhat, Rania Hefaiedh, Christian Ruiz, Luigi M. Terracciano, Richard P. Tucker, Lotfi Hendaoui, Ruth Chiquet-Ehrismann

Research output: Contribution to journalArticlepeer-review

Abstract

Extrahepatic cancers of the biliary system are typically asymptomatic until after metastasis, which contributes to their poor prognosis. Here we examined intrahepatic cholangiocarcinomas (n = 8), carcinomas of perihilar bile ducts (n = 7), carcinomas of the gallbladder (n = 11) and hepatic metastasis from carcinomas of the gallbladder (n = 4) for the expression of the extracellular matrix glycoproteins tenascin-C and tenascin-W. Anti-tenascin-C and anti-tenascin-W immunoreactivity was found in all biliary tract tumors examined. Unlike tenascin-C, tenascin-W was not detected in normal hepatobiliary tissue. Tenascin-W was also expressed by the cholangiocarcinoma-derived cell line Huh-28. However, co-culture of Huh-28 cells with immortalized bone marrow-derived stromal cells was necessary for the formation and organization of tenascin-W fibrils in vitro. Our results indicate that tenascin-W may be a novel marker of hepatobiliary tumor stroma, and its absence from many normal tissues suggests that it may be a potential target for biotherapies.

Original languageEnglish (US)
Article number630139
JournalFrontiers in immunology
Volume11
DOIs
StatePublished - Feb 22 2021

Keywords

  • extracellular matrix
  • gallbladder cancer
  • tenascin-C
  • tenascin-N
  • tenascin-W
  • tumor stroma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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