TY - JOUR
T1 - Tenascin-W Is a Novel Stromal Marker in Biliary Tract Cancers
AU - Hendaoui, Ismaïl
AU - Lahmar, Ahlem
AU - Campo, Luca
AU - Mebarki, Sihem
AU - Bichet, Sandrine
AU - Hess, Daniel
AU - Degen, Martin
AU - Kchir, Nidhameddine
AU - Charrada-Ben Farhat, Leila
AU - Hefaiedh, Rania
AU - Ruiz, Christian
AU - Terracciano, Luigi M.
AU - Tucker, Richard P.
AU - Hendaoui, Lotfi
AU - Chiquet-Ehrismann, Ruth
N1 - Funding Information:
We are grateful to Prof. Alexander Knuth (University Hospital of Z?rich, Switzerland) for generously providing cell lines. We thank the following people from Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland: Augustyn Bogucki for technical help with histology, Hubertus Kohler for performing cell sorting experiments, Laurent Gelman for assisting with imaging, and Prof. Patrick Matthias and Prof. Nancy E. Hynes for their scientific expertise and review of the manuscript. We thank the following people from Department of Surgery, Medical School, University of Tunis El Manar, Tunisia: Prof. Zoubeir Ben Safta and Prof. Mohamed Tahar Khalfallah for their clinical expertise, and Prof. Chadli Dziri for his review of the manuscript. We thank Aur?lien Lacombe (Institute of Pathology, University Hospital of Basel, Basel, Switzerland) for technical help with FFPE sections. We thank Mounira Ben Mustapha and Khadija Ben Hmida-Saad (Mongi Slim University Hospital, La Marsa, Tunisia) for administrative assistance.
Publisher Copyright:
© Copyright © 2021 Hendaoui, Lahmar, Campo, Mebarki, Bichet, Hess, Degen, Kchir, Charrada-Ben Farhat, Hefaiedh, Ruiz, Terracciano, Tucker, Hendaoui and Chiquet-Ehrismann.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/2/22
Y1 - 2021/2/22
N2 - Extrahepatic cancers of the biliary system are typically asymptomatic until after metastasis, which contributes to their poor prognosis. Here we examined intrahepatic cholangiocarcinomas (n = 8), carcinomas of perihilar bile ducts (n = 7), carcinomas of the gallbladder (n = 11) and hepatic metastasis from carcinomas of the gallbladder (n = 4) for the expression of the extracellular matrix glycoproteins tenascin-C and tenascin-W. Anti-tenascin-C and anti-tenascin-W immunoreactivity was found in all biliary tract tumors examined. Unlike tenascin-C, tenascin-W was not detected in normal hepatobiliary tissue. Tenascin-W was also expressed by the cholangiocarcinoma-derived cell line Huh-28. However, co-culture of Huh-28 cells with immortalized bone marrow-derived stromal cells was necessary for the formation and organization of tenascin-W fibrils in vitro. Our results indicate that tenascin-W may be a novel marker of hepatobiliary tumor stroma, and its absence from many normal tissues suggests that it may be a potential target for biotherapies.
AB - Extrahepatic cancers of the biliary system are typically asymptomatic until after metastasis, which contributes to their poor prognosis. Here we examined intrahepatic cholangiocarcinomas (n = 8), carcinomas of perihilar bile ducts (n = 7), carcinomas of the gallbladder (n = 11) and hepatic metastasis from carcinomas of the gallbladder (n = 4) for the expression of the extracellular matrix glycoproteins tenascin-C and tenascin-W. Anti-tenascin-C and anti-tenascin-W immunoreactivity was found in all biliary tract tumors examined. Unlike tenascin-C, tenascin-W was not detected in normal hepatobiliary tissue. Tenascin-W was also expressed by the cholangiocarcinoma-derived cell line Huh-28. However, co-culture of Huh-28 cells with immortalized bone marrow-derived stromal cells was necessary for the formation and organization of tenascin-W fibrils in vitro. Our results indicate that tenascin-W may be a novel marker of hepatobiliary tumor stroma, and its absence from many normal tissues suggests that it may be a potential target for biotherapies.
KW - extracellular matrix
KW - gallbladder cancer
KW - tenascin-C
KW - tenascin-N
KW - tenascin-W
KW - tumor stroma
UR - http://www.scopus.com/inward/record.url?scp=85102292508&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85102292508&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2020.630139
DO - 10.3389/fimmu.2020.630139
M3 - Article
C2 - 33692777
AN - SCOPUS:85102292508
VL - 11
JO - Frontiers in Immunology
JF - Frontiers in Immunology
SN - 1664-3224
M1 - 630139
ER -