Bone marrow megakaryocytes reside on a complex extracellular matrix, produced by stromal cells, as well as by megakaryocytes themselves. Using immunofluorescence microscopy we have determined the distribution of tenascin-C in guinea-pig bone marrow, finding that it has a prominent fibrillar distribution in association with megakaryocytes. Western blot analysis of CHRF-288 cells, a human megakaryocytic cell line and isolated purified guinea-pig megakaryocytes, with polyclonal and monoclonal antibodies, demonstrate the presence of three tenascin isoforms (210, 220 and 250 kDa). Immunoprecipitation of radiolabelled isolated guinea-pig megakaryocytes cultured on a type I collagen gel, demonstrate that these three tenascin isoforms were present in mega karyocytes, whereas only two isoforms were secreted into the media (210 and 220 kDa). Analysis of rat bone marrow by in situ hybridisation reveals that transcripts for tenascin-C are present in megakaryocytes. Integrin regulation of CHRF cell adhesion to intact tenascin was shown to be mediated solely by the β1 integrin.
|Original language||English (US)|
|Number of pages||8|
|Journal||Comparative Haematology International|
|State||Published - 1998|
- CHRF cells
- Extracellular matrix
ASJC Scopus subject areas