Tenascin-C is synthesised and secreted by megakaryocytes, whose adherence to intact tenascin is mediated by the integrin subunit β1

Fern Tablin, M. J. Rabier, N. J. Walker, V. M. Velasco, C. L. Field, R. M. Leven

Research output: Contribution to journalArticle

Abstract

Bone marrow megakaryocytes reside on a complex extracellular matrix, produced by stromal cells, as well as by megakaryocytes themselves. Using immunofluorescence microscopy we have determined the distribution of tenascin-C in guinea-pig bone marrow, finding that it has a prominent fibrillar distribution in association with megakaryocytes. Western blot analysis of CHRF-288 cells, a human megakaryocytic cell line and isolated purified guinea-pig megakaryocytes, with polyclonal and monoclonal antibodies, demonstrate the presence of three tenascin isoforms (210, 220 and 250 kDa). Immunoprecipitation of radiolabeled isolated guinea-pig megakaryocytes cultured on a type I collagen gel, demonstrate that these three tenascin isoforms were present in megakaryocytes, whereas only two isoforms were secreted into the media (210 and 220 kDa). Analysis of rat bone marrow by in situ hybridisation reveals that transcripts for tenascin-C are present in megakaryocytes. Integrin regulation of CHRF cell adhesion to intact tenascin was shown to be mediated solely by the β1 integrin.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalComparative Clinical Pathology
Volume8
Issue number3
StatePublished - 1998

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Tenascin
Megakaryocytes
Integrins
Protein Isoforms
Guinea Pigs
Bone Marrow
Stromal Cells
Collagen Type I
Fluorescence Microscopy
Immunoprecipitation
Cell Adhesion
In Situ Hybridization
Extracellular Matrix
Western Blotting
Gels
Monoclonal Antibodies
Cell Line

Keywords

  • CHRF cells
  • Extracellular matrix
  • Integrins
  • Megakaryocytes
  • Tenascin-C

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Tenascin-C is synthesised and secreted by megakaryocytes, whose adherence to intact tenascin is mediated by the integrin subunit β1 . / Tablin, Fern; Rabier, M. J.; Walker, N. J.; Velasco, V. M.; Field, C. L.; Leven, R. M.

In: Comparative Clinical Pathology, Vol. 8, No. 3, 1998, p. 142-149.

Research output: Contribution to journalArticle

Tablin, Fern ; Rabier, M. J. ; Walker, N. J. ; Velasco, V. M. ; Field, C. L. ; Leven, R. M. / Tenascin-C is synthesised and secreted by megakaryocytes, whose adherence to intact tenascin is mediated by the integrin subunit β1 In: Comparative Clinical Pathology. 1998 ; Vol. 8, No. 3. pp. 142-149.
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AU - Rabier, M. J.

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AU - Velasco, V. M.

AU - Field, C. L.

AU - Leven, R. M.

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