Temporal and spatial profiles of ABCA2-expressing oligodendrocytes in the developing rat brain

Yukiko Tanaka, Katsuya Yamada, Chengji Zhou, Nobuhiro Ban, Seiji Shioda, Nobuya Inagaki

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

ABCA2 protein belongs to the ABCA subclass of ATP-binding cassette (ABC) transporters proposed to exert critical functions in transmembrane transport of endogenous lipids. In this study, we found by immunoblot analyses that approximately 260 kDa of ABCA2 protein is expressed predominantly in oligodendrocytes, and that the expression of the protein is upregulated in the brain during maturation, especially between postnatal days 6 and 19. Parallel to the changes in expression of ABCA2, immunohistochemical analyses showed rapid spatial spread of ABCA2-immunolabeled oligodendrocytes in the brain during this period. These temporal and spatial changes in ABCA2 expression were in good agreement with findings in myeloarchitectonics reported previously. Further, double immunolabeling with ABCA2 and a major structural protein of myelin, myelin basic protein, demonstrated that onset of ABCA2 expression in oligodendrocytes coincides with the appearance of thick myelin segments immunolabeled with myelin basic protein. Because ABCA2 was abundantly expressed in adult cortex in white matter and gray matter, coexpression of ABCA2 and a marker for the oligodendroglial progenitors NG2 or platelet-derived growth factor α receptor was investigated. No cells coexpressing ABCA2 and the marker were observed, suggesting that ABCA2 is expressed predominantly in myelin-forming oligodendrocytes distinct from the adult oligodendroglial progenitors tested. These results suggested a role for ABCA2 in membrane transport of substrates such as the lipids that are closely linked to myelination processes.

Original languageEnglish (US)
Pages (from-to)353-367
Number of pages15
JournalJournal of Comparative Neurology
Volume455
Issue number3
DOIs
StatePublished - Jan 13 2003
Externally publishedYes

Fingerprint

Oligodendroglia
Myelin Basic Protein
Brain
Myelin Sheath
Myelin Proteins
Lipids
Platelet-Derived Growth Factor Receptors
Proteins
ATP-Binding Cassette Transporters
Membranes

Keywords

  • ATP-binding cassette transporter
  • Glial progenitors
  • Lipid transport
  • Lysosome
  • Myelin
  • Neocortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Temporal and spatial profiles of ABCA2-expressing oligodendrocytes in the developing rat brain. / Tanaka, Yukiko; Yamada, Katsuya; Zhou, Chengji; Ban, Nobuhiro; Shioda, Seiji; Inagaki, Nobuya.

In: Journal of Comparative Neurology, Vol. 455, No. 3, 13.01.2003, p. 353-367.

Research output: Contribution to journalArticle

Tanaka, Yukiko ; Yamada, Katsuya ; Zhou, Chengji ; Ban, Nobuhiro ; Shioda, Seiji ; Inagaki, Nobuya. / Temporal and spatial profiles of ABCA2-expressing oligodendrocytes in the developing rat brain. In: Journal of Comparative Neurology. 2003 ; Vol. 455, No. 3. pp. 353-367.
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