Temporal and spatial expression of transforming growth factor-β after airway remodeling to tobacco smoke in rats

Laura L. Hoang, Yen P. Nguyen, Rayza Aspeé, Sarah J. Bolton, Yi Hsin Shen, Lei Wang, Nicholas Kenyon, Suzette Smiley-Jewell, Kent E Pinkerton

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Airway remodeling is strongly correlated with the progression of chronic obstructive pulmonary disease (COPD). In this study, our goal was to characterize progressive structural changes in site-specific airways, along with the temporal and spatial expression of transforming growth factor (TGF)-b in the lungs of male spontaneously hypertensive rats exposed to tobacco smoke (TS). Our studies demonstrated that TS-induced changes of the airways is dependent on airway generation and exposure duration for proximal, midlevel, and distal airways. Stratified squamous epithelial cell metaplasia was evident in the most proximal airways after 4 and 12 weeks but with minimal levels of TGF-β-positive epithelial cells after only 4 weeks of exposure. In contrast, epithelial cells in midlevel and distal airways were strongly TGF-β positive at both 4 and 12 weeks of TS exposure. Airway smooth muscle volume increased significantly at 4 and 12 weeks in midlevel airways. Immunohistochemistry of TGF-β was also found to be significantly increased at 4 and 12 weeks in lymphoid tissues and alveolar macrophages. ELISA of whole-lung homogenate demonstrated that TGF-β2 was increased after 4 and 12 weeks of TS exposure, whereas TGF-β1 was decreased at 12 weeks of TS exposure. Airway levels of messenger RNA for TGF-β2, as well as platelet-derived growth factor-A, granulocyte-macrophage colony-stimulating factor, and vascular endothelial growth factor-a, growth factors regulated by TGF-β, were significantly decreased in animals after 12 weeks of TS exposure. Our data indicate that TS increases TGF-β in epithelial and inflammatory cells in connection with airway remodeling, although the specific role of each TGF-β isoform remains to be defined in TS-induced airway injury and disease.

Original languageEnglish (US)
Pages (from-to)872-881
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume54
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Airway Remodeling
Tobacco
Transforming Growth Factors
Smoke
Rats
Epithelial Cells
Lung
Alveolar Macrophages
Metaplasia
Lymphoid Tissue
Inbred SHR Rats
Granulocyte-Macrophage Colony-Stimulating Factor
Pulmonary diseases
Chronic Obstructive Pulmonary Disease
Vascular Endothelial Growth Factor A
Smooth Muscle
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • Airway epithelium
  • Chronic obstructive pulmonary disease
  • Spontaneously hypertensive rats
  • Tobacco smoke
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Temporal and spatial expression of transforming growth factor-β after airway remodeling to tobacco smoke in rats. / Hoang, Laura L.; Nguyen, Yen P.; Aspeé, Rayza; Bolton, Sarah J.; Shen, Yi Hsin; Wang, Lei; Kenyon, Nicholas; Smiley-Jewell, Suzette; Pinkerton, Kent E.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 54, No. 6, 01.06.2016, p. 872-881.

Research output: Contribution to journalArticle

Hoang, Laura L. ; Nguyen, Yen P. ; Aspeé, Rayza ; Bolton, Sarah J. ; Shen, Yi Hsin ; Wang, Lei ; Kenyon, Nicholas ; Smiley-Jewell, Suzette ; Pinkerton, Kent E. / Temporal and spatial expression of transforming growth factor-β after airway remodeling to tobacco smoke in rats. In: American Journal of Respiratory Cell and Molecular Biology. 2016 ; Vol. 54, No. 6. pp. 872-881.
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AU - Shen, Yi Hsin

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