Temporal and spatial expression of BMPs and BMP antagonists during posterolateral lumbar fusion

Yong Tang, Xiaojing Ye, Eric Otto Klineberg, Shane Curtiss, Sukanta Maitra, Munish C. Gupta

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Study Design. Quantitative gene expression analysis and immunohistochemistry were used to investigate the temporal and spatial expression of bone morphogenic proteins (BMPs) and BMP antagonists in a posterolateral spine fusion model in rabbits. Objective. To identify the expression pattern of BMPs and BMP antagonists and to determine the molecular and histologic changes of the graft and surrounding tissue during fusion. Summary Of Background Data. There are no studies on BMP antagonists during spinal fusion. Furthermore, the reciprocal interaction between bone grafts and surrounding tissue is still unknown in fusion. Methods. Eighteen New Zealand White rabbits underwent bilateral posterolateral spine fusion with autogenous bone graft. Rabbits were killed at 1, 2, 4, or 6 weeks after arthrodesis. The spinal fusions were analyzed by radiography. On the right side, specimens were collected from the outer zone over the transverse processes, the inner zone between the transverse processes, muscle surrounding bone grafts, and the transverse process. Gene expression of BMP-2, BMP-4, and BMP-7, noggin, chordin, Sox9, and Runx2 were measured by real-time polymerase chain reaction at each time point of each sample. On the left side, molecules of interest were evaluated by immunohistochemistry on tissue sections. Results. BMP-2, BMP-4, and BMP-7, noggin, and chordin were colocalized in rimming osteoblasts, osteoclasts, and chondrocytes. The outer zone demonstrated earlier bone maturation and faster increase in BMP gene expression than the inner zone. Muscle surrounding bone grafts showed significantly higher BMP expression and Runx2 activity at the early phase. BMP-positive cells were also noted around blood vessels. Conclusion. The colocalization and temporal relationship of BMPs and BMP antagonists suggests that BMP activity is tightly regulated by the antagonists during fusion. In addition, not only the decorticated transverse process, but also muscle surrounding bone grafts, is actively involved in osteogenesis during fusion.

Original languageEnglish (US)
JournalSpine
Volume36
Issue number4
DOIs
StatePublished - Feb 15 2011

Fingerprint

Bone and Bones
Proteins
Transplants
Spinal Fusion
Rabbits
Gene Expression
Muscles
Core Binding Factor Alpha 1 Subunit
Spine
Immunohistochemistry
Temporal Bone
Arthrodesis
Osteoclasts
Chondrocytes
Osteoblasts
Osteogenesis
Radiography
Blood Vessels
Real-Time Polymerase Chain Reaction

Keywords

  • animal model
  • antagonist
  • BMP
  • fusion
  • gene expression
  • immunolocalization
  • lumbar spine
  • rabbit

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Clinical Neurology

Cite this

Temporal and spatial expression of BMPs and BMP antagonists during posterolateral lumbar fusion. / Tang, Yong; Ye, Xiaojing; Klineberg, Eric Otto; Curtiss, Shane; Maitra, Sukanta; Gupta, Munish C.

In: Spine, Vol. 36, No. 4, 15.02.2011.

Research output: Contribution to journalArticle

Tang, Yong ; Ye, Xiaojing ; Klineberg, Eric Otto ; Curtiss, Shane ; Maitra, Sukanta ; Gupta, Munish C. / Temporal and spatial expression of BMPs and BMP antagonists during posterolateral lumbar fusion. In: Spine. 2011 ; Vol. 36, No. 4.
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abstract = "Study Design. Quantitative gene expression analysis and immunohistochemistry were used to investigate the temporal and spatial expression of bone morphogenic proteins (BMPs) and BMP antagonists in a posterolateral spine fusion model in rabbits. Objective. To identify the expression pattern of BMPs and BMP antagonists and to determine the molecular and histologic changes of the graft and surrounding tissue during fusion. Summary Of Background Data. There are no studies on BMP antagonists during spinal fusion. Furthermore, the reciprocal interaction between bone grafts and surrounding tissue is still unknown in fusion. Methods. Eighteen New Zealand White rabbits underwent bilateral posterolateral spine fusion with autogenous bone graft. Rabbits were killed at 1, 2, 4, or 6 weeks after arthrodesis. The spinal fusions were analyzed by radiography. On the right side, specimens were collected from the outer zone over the transverse processes, the inner zone between the transverse processes, muscle surrounding bone grafts, and the transverse process. Gene expression of BMP-2, BMP-4, and BMP-7, noggin, chordin, Sox9, and Runx2 were measured by real-time polymerase chain reaction at each time point of each sample. On the left side, molecules of interest were evaluated by immunohistochemistry on tissue sections. Results. BMP-2, BMP-4, and BMP-7, noggin, and chordin were colocalized in rimming osteoblasts, osteoclasts, and chondrocytes. The outer zone demonstrated earlier bone maturation and faster increase in BMP gene expression than the inner zone. Muscle surrounding bone grafts showed significantly higher BMP expression and Runx2 activity at the early phase. BMP-positive cells were also noted around blood vessels. Conclusion. The colocalization and temporal relationship of BMPs and BMP antagonists suggests that BMP activity is tightly regulated by the antagonists during fusion. In addition, not only the decorticated transverse process, but also muscle surrounding bone grafts, is actively involved in osteogenesis during fusion.",
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