Temporal and spatial distribution of ciliogenesis in the tracheobronchial airways of mice

Elina Toskala, Suzette M. Smiley-Jewell, Viviana J. Wong, Dustin King, Charles Plopper

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Little is known about ciliogenesis as it proceeds through the entire airway tree, from the trachea to the terminal bronchioles, especially during the postnatal period. The purpose of this study was to define the spatial and temporal (prenatal and postnatal) pattern of normal cilia development in the mouse. Three airway generations representing the entire airway tree were examined: trachea, lobar bronchi, and terminal bronchiole. Ciliated cells in lung lobe whole mounts were labeled with a fluorescent dye for confocal microscopy, and ciliated cell surface density was measured for each airway generation and age. The same samples were examined by scanning electron microscopy to verify the appearance of ciliated cells among the differentiating epithelium of the airways. Ciliated cells were first detected in the trachea and lobar bronchi at 16 days gestational age (DGA) and in the terminal bronchioles at 18 DGA. Ciliated cell surface density increased with prenatal and postnatal age at all airway levels. However, the ciliated cell surface density of the trachea and lobar bronchi was always greater compared with the terminal bronchiole. In conclusion, the study revealed that in developing tracheobronchial airways of the mouse: 1) Ciliogenesis differs temporally and spatially by airway generation; 2) Ciliated cell surface density increases with age in all airway generations, but density decreases in a proximal to distal direction; and 3) A significant portion of ciliogenesis continues after birth. This study provides a healthy basis for investigations of neonatal pulmonary disease or pollutant toxicity affecting cilia and its functions.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume289
Issue number3 33-3
DOIs
StatePublished - Sep 1 2005

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Bronchioles
Trachea
Bronchi
Cell Count
Cilia
Gestational Age
Infant, Newborn, Diseases
Fluorescent Dyes
Confocal Microscopy
Electron Scanning Microscopy
Lung Diseases
Epithelium
Parturition
Lung

Keywords

  • Cilia
  • Gestational
  • Lung
  • Postnatal

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Temporal and spatial distribution of ciliogenesis in the tracheobronchial airways of mice. / Toskala, Elina; Smiley-Jewell, Suzette M.; Wong, Viviana J.; King, Dustin; Plopper, Charles.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 289, No. 3 33-3, 01.09.2005.

Research output: Contribution to journalArticle

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