Temperature-dependent activation of neurons by continuous near-infrared laser

Shanshan Liang, Fan Yang, Cheng Zhou, Yue Wang, Shao Li, C. K. Sun, Jose Luis Puglisi, Donald M Bers, Changsen Sun, Jie Zheng

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14°C in 500 ms. Computer simulation confirmed that small temperature changes of about 5°C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.

Original languageEnglish (US)
Pages (from-to)33-42
Number of pages10
JournalCell Biochemistry and Biophysics
Volume53
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Infrared lasers
Neurons
Lasers
Chemical activation
Temperature
Voltage-Gated Potassium Channels
Laser modes
Computer Simulation
Calibration
Laser beams
Rats
Irradiation
Kinetics
Computer simulation
Electric potential

Keywords

  • Action potential
  • Activation
  • Inactivation
  • Laser
  • Temperature
  • Voltage-dependent ion channels

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Temperature-dependent activation of neurons by continuous near-infrared laser. / Liang, Shanshan; Yang, Fan; Zhou, Cheng; Wang, Yue; Li, Shao; Sun, C. K.; Puglisi, Jose Luis; Bers, Donald M; Sun, Changsen; Zheng, Jie.

In: Cell Biochemistry and Biophysics, Vol. 53, No. 1, 01.2009, p. 33-42.

Research output: Contribution to journalArticle

Liang, Shanshan ; Yang, Fan ; Zhou, Cheng ; Wang, Yue ; Li, Shao ; Sun, C. K. ; Puglisi, Jose Luis ; Bers, Donald M ; Sun, Changsen ; Zheng, Jie. / Temperature-dependent activation of neurons by continuous near-infrared laser. In: Cell Biochemistry and Biophysics. 2009 ; Vol. 53, No. 1. pp. 33-42.
@article{1da57dbd92da4b95a4884173a36817d0,
title = "Temperature-dependent activation of neurons by continuous near-infrared laser",
abstract = "Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14°C in 500 ms. Computer simulation confirmed that small temperature changes of about 5°C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.",
keywords = "Action potential, Activation, Inactivation, Laser, Temperature, Voltage-dependent ion channels",
author = "Shanshan Liang and Fan Yang and Cheng Zhou and Yue Wang and Shao Li and Sun, {C. K.} and Puglisi, {Jose Luis} and Bers, {Donald M} and Changsen Sun and Jie Zheng",
year = "2009",
month = "1",
doi = "10.1007/s12013-008-9035-2",
language = "English (US)",
volume = "53",
pages = "33--42",
journal = "Cell Biochemistry and Biophysics",
issn = "1085-9195",
publisher = "Humana Press",
number = "1",

}

TY - JOUR

T1 - Temperature-dependent activation of neurons by continuous near-infrared laser

AU - Liang, Shanshan

AU - Yang, Fan

AU - Zhou, Cheng

AU - Wang, Yue

AU - Li, Shao

AU - Sun, C. K.

AU - Puglisi, Jose Luis

AU - Bers, Donald M

AU - Sun, Changsen

AU - Zheng, Jie

PY - 2009/1

Y1 - 2009/1

N2 - Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14°C in 500 ms. Computer simulation confirmed that small temperature changes of about 5°C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.

AB - Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14°C in 500 ms. Computer simulation confirmed that small temperature changes of about 5°C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.

KW - Action potential

KW - Activation

KW - Inactivation

KW - Laser

KW - Temperature

KW - Voltage-dependent ion channels

UR - http://www.scopus.com/inward/record.url?scp=58549118422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58549118422&partnerID=8YFLogxK

U2 - 10.1007/s12013-008-9035-2

DO - 10.1007/s12013-008-9035-2

M3 - Article

C2 - 19034696

AN - SCOPUS:58549118422

VL - 53

SP - 33

EP - 42

JO - Cell Biochemistry and Biophysics

JF - Cell Biochemistry and Biophysics

SN - 1085-9195

IS - 1

ER -