Abstract
Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14°C in 500 ms. Computer simulation confirmed that small temperature changes of about 5°C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 33-42 |
| Number of pages | 10 |
| Journal | Cell Biochemistry and Biophysics |
| Volume | 53 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2009 |
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Keywords
- Action potential
- Activation
- Inactivation
- Laser
- Temperature
- Voltage-dependent ion channels
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology
Cite this
Temperature-dependent activation of neurons by continuous near-infrared laser. / Liang, Shanshan; Yang, Fan; Zhou, Cheng; Wang, Yue; Li, Shao; Sun, C. K.; Puglisi, Jose Luis; Bers, Donald M; Sun, Changsen; Zheng, Jie.
In: Cell Biochemistry and Biophysics, Vol. 53, No. 1, 01.2009, p. 33-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temperature-dependent activation of neurons by continuous near-infrared laser
AU - Liang, Shanshan
AU - Yang, Fan
AU - Zhou, Cheng
AU - Wang, Yue
AU - Li, Shao
AU - Sun, C. K.
AU - Puglisi, Jose Luis
AU - Bers, Donald M
AU - Sun, Changsen
AU - Zheng, Jie
PY - 2009/1
Y1 - 2009/1
N2 - Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14°C in 500 ms. Computer simulation confirmed that small temperature changes of about 5°C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.
AB - Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14°C in 500 ms. Computer simulation confirmed that small temperature changes of about 5°C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.
KW - Action potential
KW - Activation
KW - Inactivation
KW - Laser
KW - Temperature
KW - Voltage-dependent ion channels
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UR - http://www.scopus.com/inward/citedby.url?scp=58549118422&partnerID=8YFLogxK
U2 - 10.1007/s12013-008-9035-2
DO - 10.1007/s12013-008-9035-2
M3 - Article
C2 - 19034696
AN - SCOPUS:58549118422
VL - 53
SP - 33
EP - 42
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
SN - 1085-9195
IS - 1
ER -