Temperature-dependency of Betanodavirus infection in SSN-1 cell line

S. Ciulli, D. Gallardi, A. Scagliarini, M. Battilani, Ronald Hedrick, S. Prosperi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study examined the in vitro effects of temperature on Betanodavirus infection in the SSN-1 cell line. A Betanodavirus isolated from moribund sea bass fry Dicentrarchus labrax farmed in the Adriatic Sea and characterised as a RGNNV (Redspotted Grouper Nervous Necrosis Virus) genotype was used. Virus-infected SSN-1 cells were incubated at temperatures between 10 and 30°C and observed for cytopathic effects daily for 15 d. Cell-free and cell-associated viral growth were evaluated by 50% tissue culture infectious dose (TCID50) titration at 0, 24, 48, 72, 96, 144, 192, 240, 312 and 360 h post-infection. Virus replication was observed at all temperatures from 15 to 30°C. The optimal temperature for virus growth was 25°C. A temperature of 10°C was detrimental to the growth of the SSN-1 cells and cell death interfered with interpretations of viral growth. The isolate of Betanodavirus from Italian sea bass in this study demonstrates a different temperature range for growth compared to previous reports for related Betanodavirus strains, most likely due to an adaptation to the normal environmental temperatures of the host fish species of origin.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalDiseases of Aquatic Organisms
Volume68
Issue number3
DOIs
StatePublished - Mar 2 2006

Fingerprint

Betanodavirus
cell lines
infection
virus
temperature
bass
microbial growth
cells
viruses
cytopathogenicity
Dicentrarchus labrax
Adriatic Sea
virus replication
titration
fish fry
in vitro studies
tissue culture
cell death
ambient temperature
genotype

Keywords

  • Betanodavirus
  • Dicentrarchus labrax
  • Nervous necrosis virus
  • SSN-1 cell line
  • Temperature dependency
  • Viral encephalopathy and retinopathy
  • Viral growth

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Ciulli, S., Gallardi, D., Scagliarini, A., Battilani, M., Hedrick, R., & Prosperi, S. (2006). Temperature-dependency of Betanodavirus infection in SSN-1 cell line. Diseases of Aquatic Organisms, 68(3), 261-265. https://doi.org/10.3354/dao068261

Temperature-dependency of Betanodavirus infection in SSN-1 cell line. / Ciulli, S.; Gallardi, D.; Scagliarini, A.; Battilani, M.; Hedrick, Ronald; Prosperi, S.

In: Diseases of Aquatic Organisms, Vol. 68, No. 3, 02.03.2006, p. 261-265.

Research output: Contribution to journalArticle

Ciulli, S, Gallardi, D, Scagliarini, A, Battilani, M, Hedrick, R & Prosperi, S 2006, 'Temperature-dependency of Betanodavirus infection in SSN-1 cell line', Diseases of Aquatic Organisms, vol. 68, no. 3, pp. 261-265. https://doi.org/10.3354/dao068261
Ciulli, S. ; Gallardi, D. ; Scagliarini, A. ; Battilani, M. ; Hedrick, Ronald ; Prosperi, S. / Temperature-dependency of Betanodavirus infection in SSN-1 cell line. In: Diseases of Aquatic Organisms. 2006 ; Vol. 68, No. 3. pp. 261-265.
@article{6199142f025c47cfb75b5edc5159a625,
title = "Temperature-dependency of Betanodavirus infection in SSN-1 cell line",
abstract = "This study examined the in vitro effects of temperature on Betanodavirus infection in the SSN-1 cell line. A Betanodavirus isolated from moribund sea bass fry Dicentrarchus labrax farmed in the Adriatic Sea and characterised as a RGNNV (Redspotted Grouper Nervous Necrosis Virus) genotype was used. Virus-infected SSN-1 cells were incubated at temperatures between 10 and 30°C and observed for cytopathic effects daily for 15 d. Cell-free and cell-associated viral growth were evaluated by 50{\%} tissue culture infectious dose (TCID50) titration at 0, 24, 48, 72, 96, 144, 192, 240, 312 and 360 h post-infection. Virus replication was observed at all temperatures from 15 to 30°C. The optimal temperature for virus growth was 25°C. A temperature of 10°C was detrimental to the growth of the SSN-1 cells and cell death interfered with interpretations of viral growth. The isolate of Betanodavirus from Italian sea bass in this study demonstrates a different temperature range for growth compared to previous reports for related Betanodavirus strains, most likely due to an adaptation to the normal environmental temperatures of the host fish species of origin.",
keywords = "Betanodavirus, Dicentrarchus labrax, Nervous necrosis virus, SSN-1 cell line, Temperature dependency, Viral encephalopathy and retinopathy, Viral growth",
author = "S. Ciulli and D. Gallardi and A. Scagliarini and M. Battilani and Ronald Hedrick and S. Prosperi",
year = "2006",
month = "3",
day = "2",
doi = "10.3354/dao068261",
language = "English (US)",
volume = "68",
pages = "261--265",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
number = "3",

}

TY - JOUR

T1 - Temperature-dependency of Betanodavirus infection in SSN-1 cell line

AU - Ciulli, S.

AU - Gallardi, D.

AU - Scagliarini, A.

AU - Battilani, M.

AU - Hedrick, Ronald

AU - Prosperi, S.

PY - 2006/3/2

Y1 - 2006/3/2

N2 - This study examined the in vitro effects of temperature on Betanodavirus infection in the SSN-1 cell line. A Betanodavirus isolated from moribund sea bass fry Dicentrarchus labrax farmed in the Adriatic Sea and characterised as a RGNNV (Redspotted Grouper Nervous Necrosis Virus) genotype was used. Virus-infected SSN-1 cells were incubated at temperatures between 10 and 30°C and observed for cytopathic effects daily for 15 d. Cell-free and cell-associated viral growth were evaluated by 50% tissue culture infectious dose (TCID50) titration at 0, 24, 48, 72, 96, 144, 192, 240, 312 and 360 h post-infection. Virus replication was observed at all temperatures from 15 to 30°C. The optimal temperature for virus growth was 25°C. A temperature of 10°C was detrimental to the growth of the SSN-1 cells and cell death interfered with interpretations of viral growth. The isolate of Betanodavirus from Italian sea bass in this study demonstrates a different temperature range for growth compared to previous reports for related Betanodavirus strains, most likely due to an adaptation to the normal environmental temperatures of the host fish species of origin.

AB - This study examined the in vitro effects of temperature on Betanodavirus infection in the SSN-1 cell line. A Betanodavirus isolated from moribund sea bass fry Dicentrarchus labrax farmed in the Adriatic Sea and characterised as a RGNNV (Redspotted Grouper Nervous Necrosis Virus) genotype was used. Virus-infected SSN-1 cells were incubated at temperatures between 10 and 30°C and observed for cytopathic effects daily for 15 d. Cell-free and cell-associated viral growth were evaluated by 50% tissue culture infectious dose (TCID50) titration at 0, 24, 48, 72, 96, 144, 192, 240, 312 and 360 h post-infection. Virus replication was observed at all temperatures from 15 to 30°C. The optimal temperature for virus growth was 25°C. A temperature of 10°C was detrimental to the growth of the SSN-1 cells and cell death interfered with interpretations of viral growth. The isolate of Betanodavirus from Italian sea bass in this study demonstrates a different temperature range for growth compared to previous reports for related Betanodavirus strains, most likely due to an adaptation to the normal environmental temperatures of the host fish species of origin.

KW - Betanodavirus

KW - Dicentrarchus labrax

KW - Nervous necrosis virus

KW - SSN-1 cell line

KW - Temperature dependency

KW - Viral encephalopathy and retinopathy

KW - Viral growth

UR - http://www.scopus.com/inward/record.url?scp=33645358081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645358081&partnerID=8YFLogxK

U2 - 10.3354/dao068261

DO - 10.3354/dao068261

M3 - Article

C2 - 16610592

AN - SCOPUS:33645358081

VL - 68

SP - 261

EP - 265

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 3

ER -