Temperature and relative contributions of Ca transport systems in cardiac myocyte relaxation

José L. Puglisi, Rosana A. Bassani, José W M Bassani, Jatin N. Amin, Donald M Bers

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The relative contributions of the different Ca transport systems involved in cardiac relaxation were evaluated at 25 and 35°C in isolated rabbit, ferret, and cat ventricular myocytes during twitches, caffeine-induced contractures in normal Tyrode solution, and caffeine-induced contractures in Na- and Ca-free solution. The time course of intracellular [Ca] decline during these contractions in rabbit ventricular myocytes allowed estimates of the relative contributions of the sarcoplasmic reticulum (SR) Ca pump, Na/Ca exchange, sarcolemmal Ca pump, and the mitochondrial calcium uniporter (with the latter two considered together as 'slow mechanisms'). The percent contributions of the SR Ca pump, the Na/Ca exchange, and the slow mechanisms were 70, 27, and 3% at 25°C and 74, 23, and 3% at 35°C. Warming from 25 to 35°C decreases twitch contractions in rabbit and ferret myocytes and caffeine-induced contractures in normal Tyrode solution and Na- and Ca-free solution in all species. In contrast, in cat myocytes warming increased twitches, possibly because of a stronger effect of temperature on Ca influx. We conclude that increased temperature accelerates all of the Ca transport systems involved in relaxation. Despite large changes in each Ca transport system with warming, the relative contributions during relaxation remain similar at physiological temperature.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume270
Issue number5 39-5
StatePublished - 1996
Externally publishedYes

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Cardiac Myocytes
Muscle Cells
Contracture
Caffeine
Ferrets
Temperature
Sarcoplasmic Reticulum
Rabbits
Cats
Tyrode's solution

Keywords

  • cardiac muscle
  • mitochondria
  • Na/Ca exchanger
  • sarcolemmal Ca pump
  • sarcoplasmic reticulum

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Temperature and relative contributions of Ca transport systems in cardiac myocyte relaxation. / Puglisi, José L.; Bassani, Rosana A.; Bassani, José W M; Amin, Jatin N.; Bers, Donald M.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 270, No. 5 39-5, 1996.

Research output: Contribution to journalArticle

Puglisi, José L. ; Bassani, Rosana A. ; Bassani, José W M ; Amin, Jatin N. ; Bers, Donald M. / Temperature and relative contributions of Ca transport systems in cardiac myocyte relaxation. In: American Journal of Physiology - Heart and Circulatory Physiology. 1996 ; Vol. 270, No. 5 39-5.
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