Telomeres in ICF syndrome cells are vulnerable to DNA damage due to elevated DNA:RNA hybrids

Shira Sagie, Shir Toubiana, Stella R. Hartono, Hagar Katzir, Aya Tzur-Gilat, Shany Havazelet, Claire Francastel, Guillaume Velasco, Frederic Chedin, Sara Selig

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

DNA:RNA hybrids, nucleic acid structures with diverse physiological functions, can disrupt genome integrity when dysregulated. Human telomeres were shown to form hybrids with the lncRNA TERRA, yet the formation and distribution of these hybrids among telomeres, their regulation and their cellular effects remain elusive. Here we predict and confirm in several human cell types that DNA:RNA hybrids form at many subtelomeric and telomeric regions. We demonstrate that ICF syndrome cells, which exhibit short telomeres and elevated TERRA levels, are enriched for hybrids at telomeric regions throughout the cell cycle. Telomeric hybrids are associated with high levels of DNA damage at chromosome ends in ICF cells, which are significantly reduced with overexpression of RNase H1. Our findings suggest that abnormally high TERRA levels in ICF syndrome lead to accumulation of telomeric hybrids that, in turn, can result in telomeric dysfunction.

Original languageEnglish (US)
Article number14015
JournalNature Communications
Volume8
DOIs
StatePublished - Jan 24 2017

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telomeres
Telomere
DNA Damage
deoxyribonucleic acid
RNA
damage
DNA
cells
Cells
Long Noncoding RNA
genome
chromosomes
nucleic acids
Chromosomes
integrity
Nucleic Acids
Genes
cycles
Cell Cycle
Genome

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Sagie, S., Toubiana, S., Hartono, S. R., Katzir, H., Tzur-Gilat, A., Havazelet, S., ... Selig, S. (2017). Telomeres in ICF syndrome cells are vulnerable to DNA damage due to elevated DNA:RNA hybrids. Nature Communications, 8, [14015]. https://doi.org/10.1038/ncomms14015

Telomeres in ICF syndrome cells are vulnerable to DNA damage due to elevated DNA:RNA hybrids. / Sagie, Shira; Toubiana, Shir; Hartono, Stella R.; Katzir, Hagar; Tzur-Gilat, Aya; Havazelet, Shany; Francastel, Claire; Velasco, Guillaume; Chedin, Frederic; Selig, Sara.

In: Nature Communications, Vol. 8, 14015, 24.01.2017.

Research output: Contribution to journalArticle

Sagie, S, Toubiana, S, Hartono, SR, Katzir, H, Tzur-Gilat, A, Havazelet, S, Francastel, C, Velasco, G, Chedin, F & Selig, S 2017, 'Telomeres in ICF syndrome cells are vulnerable to DNA damage due to elevated DNA:RNA hybrids', Nature Communications, vol. 8, 14015. https://doi.org/10.1038/ncomms14015
Sagie S, Toubiana S, Hartono SR, Katzir H, Tzur-Gilat A, Havazelet S et al. Telomeres in ICF syndrome cells are vulnerable to DNA damage due to elevated DNA:RNA hybrids. Nature Communications. 2017 Jan 24;8. 14015. https://doi.org/10.1038/ncomms14015
Sagie, Shira ; Toubiana, Shir ; Hartono, Stella R. ; Katzir, Hagar ; Tzur-Gilat, Aya ; Havazelet, Shany ; Francastel, Claire ; Velasco, Guillaume ; Chedin, Frederic ; Selig, Sara. / Telomeres in ICF syndrome cells are vulnerable to DNA damage due to elevated DNA:RNA hybrids. In: Nature Communications. 2017 ; Vol. 8.
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