Telomerase activity, telomere length, and DNA ploidy in prostatic intraepithelial neoplasia (PIN)

Kenneth S. Koeneman, Chong-Xian Pan, Jia Kuan Jin, Joseph M. Pyle, Robert C. Flanigan, T. Vincent Shankey, Manuel O. Diaz

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Purpose: To investigate the relationship of telomerase activity, telomere length, and DNA ploidy in high grade prostatic intraepithelial neoplasia (PIN). Materials and Methods: Tissue samples were carefully microdissected to obtain adenocarcinoma or PIN-containing tissue free of cancer. Telomerase activity was measured using the PCR-based telomeric repeat amplification protocol (TRAP). Telomere length was estimated from Southern blots of telomere restriction fragments (TRFs). DNA ploidy of PIN and carcinoma was determined by image analysis of adjacent Feulgen stained tissue sections. Results: Telomerase activity was found in 4 of 25 samples (16%) of high grade PIN. All telomerase positive PIN foci had a diploid DNA content. Although 5 of 25 samples (25%) of high grade PIN foci analyzed were DNA aneuploid, none of these demonstrated telomerase activity. Telomerase positive foci of prostate carcinoma (69% of all cancer foci analyzed) displayed heterogeneity in TRF length, with a mean TRF length two kilobase pairs shorter than that of telomerase negative specimens. Conclusions: Telomerase activity is present in a low percentage of high-grade PIN foci, which are diploid by DNA content measurements.

Original languageEnglish (US)
Pages (from-to)1533-1539
Number of pages7
JournalJournal of Urology
Volume160
Issue number4
DOIs
StatePublished - Oct 1998
Externally publishedYes

Keywords

  • DNA ploidy
  • Prostatic intraepithelial neoplasia
  • Telomerase
  • Telomere length

ASJC Scopus subject areas

  • Urology

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  • Cite this

    Koeneman, K. S., Pan, C-X., Jin, J. K., Pyle, J. M., Flanigan, R. C., Shankey, T. V., & Diaz, M. O. (1998). Telomerase activity, telomere length, and DNA ploidy in prostatic intraepithelial neoplasia (PIN). Journal of Urology, 160(4), 1533-1539. https://doi.org/10.1016/S0022-5347(01)62608-9