Techniques for gene transfer into neurons

Philip Washbourne, A Kimberley Usrey

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

To illuminate the function of the thousands of genes that make up the complexity of the nervous system, it is critical to be able to introduce and express DNA in neurons. Over the past two decades, many gene transfer methods have been developed, including viral vectors, liposomes and electroporation. Although the perfect gene transfer technique for every application has not yet been developed, recent technical advances have facilitated the ease of neuronal gene transfer and have increased the accessibility of these techniques to all laboratories. In order to select a transfection method for any particular experiment, the specific advantages and disadvantages of each technique must be considered.

Original languageEnglish (US)
Pages (from-to)566-573
Number of pages8
JournalCurrent Opinion in Neurobiology
Volume12
Issue number5
DOIs
StatePublished - Oct 1 2002

ASJC Scopus subject areas

  • Neuroscience(all)

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