Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS)

Dinglong Ma, Julien Bec, Dimitris Gorpas, Diego Yankelevich, Laura Marcu

Research output: Contribution to journalArticle

17 Scopus citations


We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).

Original languageEnglish (US)
Pages (from-to)987-1002
Number of pages16
JournalBiomedical Optics Express
Issue number3
StatePublished - 2015


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

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