Abstract
We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 987-1002 |
| Number of pages | 16 |
| Journal | Biomedical Optics Express |
| Volume | 6 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2015 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Biotechnology
Cite this
Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS). / Ma, Dinglong; Bec, Julien; Gorpas, Dimitris; Yankelevich, Diego; Marcu, Laura.
In: Biomedical Optics Express, Vol. 6, No. 3, 2015, p. 987-1002.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS)
AU - Ma, Dinglong
AU - Bec, Julien
AU - Gorpas, Dimitris
AU - Yankelevich, Diego
AU - Marcu, Laura
PY - 2015
Y1 - 2015
N2 - We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).
AB - We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).
UR - http://www.scopus.com/inward/record.url?scp=84942374100&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942374100&partnerID=8YFLogxK
U2 - 10.1364/BOE.6.000987
DO - 10.1364/BOE.6.000987
M3 - Article
AN - SCOPUS:84942374100
VL - 6
SP - 987
EP - 1002
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 3
ER -