Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS)

Dinglong Ma, Julien Bec, Dimitris Gorpas, Diego Yankelevich, Laura Marcu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).

Original languageEnglish (US)
Pages (from-to)987-1002
Number of pages16
JournalBiomedical Optics Express
Volume6
Issue number3
DOIs
StatePublished - 2015

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Fluorescence Spectrometry
Fluorescence
life (durability)
fluorescence
scanning
spectroscopy
Biopsy
Photons
delineation
Software
Hand
markers
discrimination
margins
fiber optics
acquisition
platforms
computer programs
probes
photons

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS). / Ma, Dinglong; Bec, Julien; Gorpas, Dimitris; Yankelevich, Diego; Marcu, Laura.

In: Biomedical Optics Express, Vol. 6, No. 3, 2015, p. 987-1002.

Research output: Contribution to journalArticle

Ma, Dinglong ; Bec, Julien ; Gorpas, Dimitris ; Yankelevich, Diego ; Marcu, Laura. / Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS). In: Biomedical Optics Express. 2015 ; Vol. 6, No. 3. pp. 987-1002.
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