TCDD suppression of tissue transglutaminase stimulation by retinoids in malignant human keratinocytes

Sheryl R. Krig, Robert H. Rice

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The human keratinocyte line SCC-4 is a model system in which to explore the mechanism by which 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) interferes with the action of hormones in the steroid receptor superfamily. In present work, retinoid induction of tissue transglutaminase mRNA was suppressed 60- 70% by 10 nM TCDD in the human squamous carcinoma cell line SCC-4. This effect occurred without enhanced degradation of the mRNA and thus appeared to result from altered transcription. The actions of all-trans-retinoic acid and the synthetic retinoid TTNPB ((E)-4-[2-(5,6,7,8-tetrahydro-5,5,8,8- tetramethyl-2-naphthylenyl)-1-propenyl] benzoic acid), which resists metabolic degradation, were suppressed to the same extent without obvious changes in their EC50s. In addition, TCDD suppression of reporter transcription, driven by a retinoic acid response element, was not evident in transient or stable transfections of SCC-4 cells. Sodium butyrate (3 mM) alone induced tissue transglutaminase and augmented retinoid induction. In the presence of butyrate, TCDD acted as an inducer and did not reduce retinoid stimulation. Retinoic acid induction of tissue transglutaminase displayed a lag phase of > 24 h, indicating that the induction has an indirect component. Rather than depleting active retinoid in the culture medium or generally inactivating retinoid receptor function, TCDD may suppress retinoid action in this case by interfering with the late phase of induction.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalToxicological Sciences
Volume56
Issue number2
StatePublished - 2000

Fingerprint

Retinoids
Keratinocytes
Tretinoin
Transcription
Degradation
Messenger RNA
Benzoic Acid
Butyric Acid
Butyrates
Steroid Receptors
RNA Stability
Response Elements
transglutaminase 2
Polychlorinated Dibenzodioxins
1,4-dioxin
Transfection
Culture Media
Squamous Cell Carcinoma
Hormones
Cell Line

Keywords

  • (E)-4-[2- (5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2- naphthylenyl)-1-propenyl] benzoic acid (TTNPB)
  • Ah receptor
  • Butyrate
  • Dioxin
  • Tissue transglutaminase (TGM2)

ASJC Scopus subject areas

  • Toxicology

Cite this

TCDD suppression of tissue transglutaminase stimulation by retinoids in malignant human keratinocytes. / Krig, Sheryl R.; Rice, Robert H.

In: Toxicological Sciences, Vol. 56, No. 2, 2000, p. 357-364.

Research output: Contribution to journalArticle

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