TASR-1 regulates alternative splicing of collagen genes in chondrogenic cells

Hiroshi Matsushita, Michael L. Blackburn, Eric Otto Klineberg, Anna Zielinska-Kwiatkowska, Mark E. Bolander, Gobinda Sarkar, Larry J. Suva, Howard A. Chansky, Liu Yang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During the differentiation of chondroprogenitors into mature chondrocytes, the alternative splicing of collagen genes switches from longer isoforms to shorter ones. To investigate the underlying mechanisms, we infected mouse ATDC5 chondroprogenitor cells with retrovirus for stable expression of two closely related SR splicing factors. RT-PCR analysis revealed that TASR-1, but not TASR-2, influenced alternative splicing of type II and type XI collagens in ATDC5 cells. The effect of TASR-1 on splicing could be reversed with the addition of insulin. Results from our microarray analysis of ATDC5 cells showed that TASR-1 and TASR-2 differentially affect genes involved in the differentiation of chondrocytes. Of special interest is the finding that TASR-1 could down-regulate expression of type X collagen, a hallmark of hypertrophic chondrocytes. Immunohistostaining demonstrated that TASR-1 protein is more abundantly expressed than TASR-2 in mouse articular chondrocytes, raising the possibility that TASR-1 might be involved in phenotype maintenance of articular chondrocytes.

Original languageEnglish (US)
Pages (from-to)411-417
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number2
DOIs
StatePublished - May 4 2007
Externally publishedYes

Fingerprint

Alternative Splicing
Chondrocytes
Collagen Type XI
Collagen
Genes
Collagen Type X
Microarrays
Protein Isoforms
Switches
Insulin
Joints
Switch Genes
Tissue Array Analysis
Collagen Type II
Retroviridae
Proteins
Down-Regulation
Maintenance
Phenotype
Polymerase Chain Reaction

Keywords

  • Articular chondrocyte
  • Chondrocyte differentiation
  • Chondrogenesis
  • Collagen genes
  • Splicing factor
  • SR protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Matsushita, H., Blackburn, M. L., Klineberg, E. O., Zielinska-Kwiatkowska, A., Bolander, M. E., Sarkar, G., ... Yang, L. (2007). TASR-1 regulates alternative splicing of collagen genes in chondrogenic cells. Biochemical and Biophysical Research Communications, 356(2), 411-417. https://doi.org/10.1016/j.bbrc.2007.02.159

TASR-1 regulates alternative splicing of collagen genes in chondrogenic cells. / Matsushita, Hiroshi; Blackburn, Michael L.; Klineberg, Eric Otto; Zielinska-Kwiatkowska, Anna; Bolander, Mark E.; Sarkar, Gobinda; Suva, Larry J.; Chansky, Howard A.; Yang, Liu.

In: Biochemical and Biophysical Research Communications, Vol. 356, No. 2, 04.05.2007, p. 411-417.

Research output: Contribution to journalArticle

Matsushita, H, Blackburn, ML, Klineberg, EO, Zielinska-Kwiatkowska, A, Bolander, ME, Sarkar, G, Suva, LJ, Chansky, HA & Yang, L 2007, 'TASR-1 regulates alternative splicing of collagen genes in chondrogenic cells', Biochemical and Biophysical Research Communications, vol. 356, no. 2, pp. 411-417. https://doi.org/10.1016/j.bbrc.2007.02.159
Matsushita, Hiroshi ; Blackburn, Michael L. ; Klineberg, Eric Otto ; Zielinska-Kwiatkowska, Anna ; Bolander, Mark E. ; Sarkar, Gobinda ; Suva, Larry J. ; Chansky, Howard A. ; Yang, Liu. / TASR-1 regulates alternative splicing of collagen genes in chondrogenic cells. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 356, No. 2. pp. 411-417.
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