Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors

Masayuki Akimoto, Hong Cheng, Dongxiao Zhu, Joseph A. Brzezinski, Ritu Khanna, Elena Filippova, Edwin C T Oh, Yuezhou Jing, Jose Luis Linares, Matthew Brooks, Sepideh Zareparsi, Alan J. Mears, Alfred Hero, Thomas M Glaser, Anand Swaroop

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation of the Nrl gene in mice leads to functional cones at the expense of rods. We show that a 2.5-kb Nrl promoter segment directs the expression of enhanced GFP specifically to rod photoreceptors and the pineal gland of transgenic mice, GFP is detected shortly after terminal cell division, corresponding to the timing of rod genesis revealed by birthdating studies. In Nrl-/- retinas, the GFP+ photoreceptors express S-opsin, consistent with the transformation of rod precursors into cones. We report the gene profiles of freshly isolated flow-sorted GFP+ photoreceptors from wild-type and Nrl-/- retinas at five distinct developmental stages. Our results provide a framework for establishing gene regulatory networks that lead to mature functional photoreceptors from postmitotic precursors. Differentially expressed rod and cone genes are excellent candidates for retinopathies.

Original languageEnglish (US)
Pages (from-to)3890-3895
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number10
DOIs
StatePublished - Mar 7 2006
Externally publishedYes

Fingerprint

Retina
Maf Transcription Factors
Genes
Opsins
Retinal Rod Photoreceptor Cells
Vertebrate Photoreceptor Cells
Pineal Gland
Gene Regulatory Networks
Cell Division
Transgenic Mice
Mammals

Keywords

  • Gene profiling
  • Gene regulation
  • Neuronal differentiation
  • Retina
  • Transcription factor

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors. / Akimoto, Masayuki; Cheng, Hong; Zhu, Dongxiao; Brzezinski, Joseph A.; Khanna, Ritu; Filippova, Elena; Oh, Edwin C T; Jing, Yuezhou; Linares, Jose Luis; Brooks, Matthew; Zareparsi, Sepideh; Mears, Alan J.; Hero, Alfred; Glaser, Thomas M; Swaroop, Anand.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 10, 07.03.2006, p. 3890-3895.

Research output: Contribution to journalArticle

Akimoto, M, Cheng, H, Zhu, D, Brzezinski, JA, Khanna, R, Filippova, E, Oh, ECT, Jing, Y, Linares, JL, Brooks, M, Zareparsi, S, Mears, AJ, Hero, A, Glaser, TM & Swaroop, A 2006, 'Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 10, pp. 3890-3895. https://doi.org/10.1073/pnas.0508214103
Akimoto, Masayuki ; Cheng, Hong ; Zhu, Dongxiao ; Brzezinski, Joseph A. ; Khanna, Ritu ; Filippova, Elena ; Oh, Edwin C T ; Jing, Yuezhou ; Linares, Jose Luis ; Brooks, Matthew ; Zareparsi, Sepideh ; Mears, Alan J. ; Hero, Alfred ; Glaser, Thomas M ; Swaroop, Anand. / Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 10. pp. 3890-3895.
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