Abstract
The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation of the Nrl gene in mice leads to functional cones at the expense of rods. We show that a 2.5-kb Nrl promoter segment directs the expression of enhanced GFP specifically to rod photoreceptors and the pineal gland of transgenic mice, GFP is detected shortly after terminal cell division, corresponding to the timing of rod genesis revealed by birthdating studies. In Nrl-/- retinas, the GFP+ photoreceptors express S-opsin, consistent with the transformation of rod precursors into cones. We report the gene profiles of freshly isolated flow-sorted GFP+ photoreceptors from wild-type and Nrl-/- retinas at five distinct developmental stages. Our results provide a framework for establishing gene regulatory networks that lead to mature functional photoreceptors from postmitotic precursors. Differentially expressed rod and cone genes are excellent candidates for retinopathies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3890-3895 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 103 |
| Issue number | 10 |
| DOIs | |
| State | Published - Mar 7 2006 |
| Externally published | Yes |
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Keywords
- Gene profiling
- Gene regulation
- Neuronal differentiation
- Retina
- Transcription factor
ASJC Scopus subject areas
- Genetics
- General
Cite this
Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors. / Akimoto, Masayuki; Cheng, Hong; Zhu, Dongxiao; Brzezinski, Joseph A.; Khanna, Ritu; Filippova, Elena; Oh, Edwin C T; Jing, Yuezhou; Linares, Jose Luis; Brooks, Matthew; Zareparsi, Sepideh; Mears, Alan J.; Hero, Alfred; Glaser, Thomas M; Swaroop, Anand.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 10, 07.03.2006, p. 3890-3895.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors
AU - Akimoto, Masayuki
AU - Cheng, Hong
AU - Zhu, Dongxiao
AU - Brzezinski, Joseph A.
AU - Khanna, Ritu
AU - Filippova, Elena
AU - Oh, Edwin C T
AU - Jing, Yuezhou
AU - Linares, Jose Luis
AU - Brooks, Matthew
AU - Zareparsi, Sepideh
AU - Mears, Alan J.
AU - Hero, Alfred
AU - Glaser, Thomas M
AU - Swaroop, Anand
PY - 2006/3/7
Y1 - 2006/3/7
N2 - The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation of the Nrl gene in mice leads to functional cones at the expense of rods. We show that a 2.5-kb Nrl promoter segment directs the expression of enhanced GFP specifically to rod photoreceptors and the pineal gland of transgenic mice, GFP is detected shortly after terminal cell division, corresponding to the timing of rod genesis revealed by birthdating studies. In Nrl-/- retinas, the GFP+ photoreceptors express S-opsin, consistent with the transformation of rod precursors into cones. We report the gene profiles of freshly isolated flow-sorted GFP+ photoreceptors from wild-type and Nrl-/- retinas at five distinct developmental stages. Our results provide a framework for establishing gene regulatory networks that lead to mature functional photoreceptors from postmitotic precursors. Differentially expressed rod and cone genes are excellent candidates for retinopathies.
AB - The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation of the Nrl gene in mice leads to functional cones at the expense of rods. We show that a 2.5-kb Nrl promoter segment directs the expression of enhanced GFP specifically to rod photoreceptors and the pineal gland of transgenic mice, GFP is detected shortly after terminal cell division, corresponding to the timing of rod genesis revealed by birthdating studies. In Nrl-/- retinas, the GFP+ photoreceptors express S-opsin, consistent with the transformation of rod precursors into cones. We report the gene profiles of freshly isolated flow-sorted GFP+ photoreceptors from wild-type and Nrl-/- retinas at five distinct developmental stages. Our results provide a framework for establishing gene regulatory networks that lead to mature functional photoreceptors from postmitotic precursors. Differentially expressed rod and cone genes are excellent candidates for retinopathies.
KW - Gene profiling
KW - Gene regulation
KW - Neuronal differentiation
KW - Retina
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=33644853775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644853775&partnerID=8YFLogxK
U2 - 10.1073/pnas.0508214103
DO - 10.1073/pnas.0508214103
M3 - Article
C2 - 16505381
AN - SCOPUS:33644853775
VL - 103
SP - 3890
EP - 3895
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 10
ER -