Targeted Measurements of O- and N-Glycopeptides Show That Proteins in High Density Lipoprotein Particles Are Enriched with Specific Glycosylation Compared to Plasma

Muchena J. Kailemia, Wanghui Wei, Khoa Nguyen, Elizabeth Beals, Lisa Sawrey-Kubicek, Christopher Rhodes, Chenghao Zhu, Romina Sacchi, Angela M. Zivkovic, Carlito B Lebrilla

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

High density lipoprotein (HDL) particles are believed to be protective due to their inverse correlation with the prevalence of cardiovascular diseases. However, recent studies show that in some conditions such as heart disease and diabetes, HDL particles can become dysfunctional. Great attention has been directed toward HDL particle composition because the relative abundances of HDL constituents determine HDL's functional properties. A key factor to consider when studying the structure and composition of plasma particles is the protein glycosylation. Here, we profile the O- and N-linked glycosylation of HDL associated-proteins including the truncated form of Apo CIII and their glycan heterogeneity in a site-specific manner. Apolipoprotein CIII, fetuin A, and alpha 1 antitrypsin are glycoproteins associated with lipoproteins and are implicated in many cardiovascular and other disease conditions. A targeted method (UHPLC-QQQ) was used to measure the glycoprotein concentrations and site-specific glycovariations of the proteins in human plasma and compared with HDL particles isolated from the same plasma samples. The proteins found in the plasma are differentially glycosylated compared to those isolated in HDL. The results of this study suggest that glycosylation may play a role in protein partitioning in the blood, with possible functional implications.

Original languageEnglish (US)
Pages (from-to)834-845
Number of pages12
JournalJournal of Proteome Research
Volume17
Issue number2
DOIs
StatePublished - Feb 2 2018

Keywords

  • Apo CIII
  • fetuin A
  • glycopeptides
  • glycosylation
  • HDL
  • high density lipoprotein
  • MRM
  • tandem mass spectrometry
  • UPLC

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Targeted Measurements of O- and N-Glycopeptides Show That Proteins in High Density Lipoprotein Particles Are Enriched with Specific Glycosylation Compared to Plasma'. Together they form a unique fingerprint.

  • Cite this