Targeted lymph-node immunization with whole inactivated simian immunodeficiency virus (SIV) or envelope and core subunit antigen vaccines does not reliably protect rhesus macaques from vaginal challenge with SIV(mac251)

X. Lu, H. Kiyono, D. Lu, S. Kawabata, J. Torten, S. Srinivasan, P. J. Dailey, J. R. McGhee, T. Lehner, Chris J Miller

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Objective: Sexual transmission of HIV is the most common route of HIV transmission throughout the world. To prevent sexually transmitted HIV infection, a vaccine is urgently needed. A previous report demonstrated the targeted immunization of the iliac lymph nodes with simian immunodeficiency virus (SIV) subunits protects rhesus macaques from rectal challenge with SIV. We sought to determine whether this immunization strategy could protect rhesus macaques from vaginal challenge with SIV. Design: Macaques were immunized with either whole-killed SIV or envelope and core subunit antigen vaccines. Using three independent groups, with three macaques in each group, macaques were immunized by the targeted iliac lymph node (TILN) route, injecting the vaccine close to the iliac lymph nodes that drain the genital tract. Results: The TILN immunization procedure induced high-titer SIV-specific immunoglobulin (Ig) G antibodies in serum in all animals and anti-SIV IgG and IgA antibodies in the cervicovaginal secretions of most animals. After a series of three or four TILN immunizations, the animals were intravaginally challenged with SIV(mac251). All animals became virus isolation-positive, except one animal immunized with SIV p27 and gp120. This animal was virus isolation-negative but SIV DNA proviral sequences were detected in peripheral blood mononuclear cells. Conclusions: In this series of studies, reliable protection from vaginal transmission of SIV was not achieved by the TILN immunization procedure.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalAIDS
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Intravaginal challenge
  • Rhesus macaques
  • Simian immunodeficiency virus
  • Targeted iliac lymph node
  • Vaginal immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Targeted lymph-node immunization with whole inactivated simian immunodeficiency virus (SIV) or envelope and core subunit antigen vaccines does not reliably protect rhesus macaques from vaginal challenge with SIV(mac251)'. Together they form a unique fingerprint.

Cite this