Tapeworm infection reduces epithelial ion transport abnormalities in murine dextran sulfate sodium-induced colitis

Colin Reardon, A. Sanchez, C. M. Hogaboam, D. M. McKay

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The rat tapeworm Hymenolepis diminuta was used to test the hypothesis that helminth infection could modulate murine colitis. Mice were infected with five H. diminuta cysticercoids, and colitis was evoked via free access to 4% (wt/vol) dextran sulfate sodium (DSS)-containing drinking water for 5 days. BALB/c mice were either infected with H. diminuta and 7 days later exposed to DSS (prophylactic strategy) or started on DSS and infected with H. diminuta 48 h later (treatment strategy). Naive and H. diminuta-only-infected mice served as controls. On autopsy, colonic segments were processed for histological examination and myeloperoxidase (MPO) measurement or mounted in Ussing chambers for assessment of epithelial ion transport. Cytokines (gamma interferon [IFN-γ], interleukin 12 [IL-12], and IL-10) were measured in serum and colonic tissue homogenates. DSS treatment resulted in reduced ion responses (indicated by short-circuit current [Isc]) to electrical nerve stimulation, the cholinergic agonist carbachol, and the adenylate cyclase activator forskolin compared to controls. H. diminuta infection, either prophylactic or therapeutic, caused a significant (P < 0.05) amelioration of these DSS-induced irregularities in stimulated ion transport. In contrast, the histopathology (i.e., mixed immune cell infiltrate, edema, and ulcerative damage) and elevated MPO levels that accompany DSS colitis were unaffected by concomitant H. diminuta infection. Similarly, there were no significant differences in levels of IFN-γ, IL-12, or IL-10 in serum or tissue from any of the treatment groups at the time of autopsy. We suggest that abolishment of colitis-induced epithelial ion transport abnormalities by H. diminuta infection provides proof-of-principle data and speculate that helminth therapy may provide relief of disease symptoms in colitis.

Original languageEnglish (US)
Pages (from-to)4417-4423
Number of pages7
JournalInfection and Immunity
Volume69
Issue number7
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Cestode Infections
Hymenolepis diminuta
Dextran Sulfate
Ion Transport
Colitis
Hymenolepiasis
Helminths
Interleukin-12
Interleukin-10
Peroxidase
Autopsy
Cholinergic Agonists
Carbachol
Colforsin
Serum
Adenylyl Cyclases
Drinking Water
Interferons
Electric Stimulation
Interferon-gamma

ASJC Scopus subject areas

  • Immunology

Cite this

Tapeworm infection reduces epithelial ion transport abnormalities in murine dextran sulfate sodium-induced colitis. / Reardon, Colin; Sanchez, A.; Hogaboam, C. M.; McKay, D. M.

In: Infection and Immunity, Vol. 69, No. 7, 2001, p. 4417-4423.

Research output: Contribution to journalArticle

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