Tandem zyxin LIM sequences do not enhance force sensitive accumulation

Amanda N. Steele, Grant M. Sumida, Soichiro Yamada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ability to sense mechanical forces is vital to cell physiology. Yet, the molecular basis of mechano-signaling remains unclear. Previous studies have shown that zyxin, a focal adhesion protein, is recruited at force-bearing sites on the actin cytoskeleton and, therefore, identifying zyxin as a mechano-sensing protein candidate. Furthermore, zyxin accumulation at force-bearing sites requires the LIM domain located at the C-terminus of zyxin. The zyxin LIM domain consists of three LIM motifs, each containing two zinc-binding sites. Since individual LIM motifs do not accumulate at focal adhesions or force-bearing sites, we hypothesize that multiple zyxin LIM domains increase force sensitivity. Using a miniature force sensor and GFP-tagged LIM variants, we quantified the relationship between single, tandem dimer and trimer LIM protein localization and traction forces. While the presence of extra LIM domains affected VASP recruitment to focal adhesions, force sensitivity was not enhanced over the single LIM domain. Therefore, zyxin force sensitivity is optimal with a single LIM domain, while additional LIM domains fail to enhance force sensitivity.

Original languageEnglish (US)
Pages (from-to)653-657
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume422
Issue number4
DOIs
StatePublished - Jun 15 2012

Fingerprint

Zyxin
Bearings (structural)
Focal Adhesions
Adhesion
Cell Physiological Phenomena
Proteins
Physiology
Traction
Actin Cytoskeleton
Dimers
Zinc
Actins
Binding Sites

Keywords

  • Cell migration
  • Focal adhesion
  • Force sensor
  • LIM domain
  • Traction force
  • Zyxin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Tandem zyxin LIM sequences do not enhance force sensitive accumulation. / Steele, Amanda N.; Sumida, Grant M.; Yamada, Soichiro.

In: Biochemical and Biophysical Research Communications, Vol. 422, No. 4, 15.06.2012, p. 653-657.

Research output: Contribution to journalArticle

Steele, Amanda N. ; Sumida, Grant M. ; Yamada, Soichiro. / Tandem zyxin LIM sequences do not enhance force sensitive accumulation. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 422, No. 4. pp. 653-657.
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