Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing

Alexander V. Mazin, Elena Zaitseva, Patrick Sung, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The repair of potentially lethal DNA double-stranded breaks (DSBs) by homologous recombination requires processing of the broken DNA into a resected DNA duplex with a protruding 3'-single-stranded DNA (ssDNA) tail. Accordingly, the canonical models for DSB repair require invasion of an intact homologous DNA template by the 3'-end of the ssDNA, a characteristic that the bacterial pairing protein RecA possesses. Unexpectedly, we find that for the eukaryotic homolog, Rad51 protein, the 5'-end of ssDNA is more invasive than the 3'-end. This pairing bias is unaffected by Rad52, Rad54 or Rad55-57 proteins. However, further investigation reveals that, in contrast to RecA protein, the preferred DNA substrate for Rad51 protein is not ssDNA but rather dsDNA with ssDNA tails. This important distinction permits the Rad51 proteins to promote DNA strand invasion using either 3'- or 5'-ends with similar efficiency.

Original languageEnglish (US)
Pages (from-to)1148-1156
Number of pages9
JournalEMBO Journal
Volume19
Issue number5
StatePublished - Mar 1 2000

Fingerprint

Single-Stranded DNA
DNA
Substrates
Rec A Recombinases
Proteins
Repair
Bacterial Proteins
Double-Stranded DNA Breaks
Homologous Recombination
Processing

Keywords

  • DNA strand exchange
  • Homologous recombination
  • Joint molecule formation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Mazin, A. V., Zaitseva, E., Sung, P., & Kowalczykowski, S. C. (2000). Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing. EMBO Journal, 19(5), 1148-1156.

Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing. / Mazin, Alexander V.; Zaitseva, Elena; Sung, Patrick; Kowalczykowski, Stephen C.

In: EMBO Journal, Vol. 19, No. 5, 01.03.2000, p. 1148-1156.

Research output: Contribution to journalArticle

Mazin, AV, Zaitseva, E, Sung, P & Kowalczykowski, SC 2000, 'Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing', EMBO Journal, vol. 19, no. 5, pp. 1148-1156.
Mazin AV, Zaitseva E, Sung P, Kowalczykowski SC. Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing. EMBO Journal. 2000 Mar 1;19(5):1148-1156.
Mazin, Alexander V. ; Zaitseva, Elena ; Sung, Patrick ; Kowalczykowski, Stephen C. / Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing. In: EMBO Journal. 2000 ; Vol. 19, No. 5. pp. 1148-1156.
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