T lymphocyte colony formation and autoimmune disease: In vitro assessment of immunopathology

D. D. Eckels, M. Eric Gershwin

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Human T lymphocyte colonies were generated with phytohemagglutinin in semi-solid media to assess the influence of cellular and humoral components upon colony formation by patients with systemic lupus erythematosus (SLE) compared to normal age-matched controls. Optimal colony formation occurred for patients with SLE and controls approximately 6 d after culture. However, colony formation by patients with active disease was significantly reduced compared to both controls and patients with inactive disease. Similar results were obtained in liquid cultures. There was no statistical correlation between colony forming capacity and quantitation of liquid culture transformation. Observation of the influences of patient sera produced striking dose response relationships. SLE sera were found to inhibit colony formation at high concentration (30-190%) but enhanced at lower concentrations (25-694%).

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalJournal of Rheumatology
Volume8
Issue number2
StatePublished - 1981

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Fingerprint Dive into the research topics of 'T lymphocyte colony formation and autoimmune disease: In vitro assessment of immunopathology'. Together they form a unique fingerprint.

  • Cite this