T-helper-cell cytokines in the early evolution of murine Lyme arthritis

Insoo Kang, Stephen W Barthold, David H. Persing, Linda K. Bockenstedt

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Genetic susceptibility to murine Lyme arthritis has been correlated with the dominance of T-helper (Th1)- or Th2-cell-associated cytokines. To determine when commitment of the Th cell phenotype occurs, we examined the kinetics of gamma interferon (IFN-γ) and interleukin-4 (IL-4) production by lymph node T cells of disease-susceptible C3H/HeN and disease-resistant BALB/c mice from days 2 through 30 of infection, a period encompassing the evolution of disease and early regression. BALB/c mice produced more IFN-γ on day 2 of infection than did C3H/HeN mice, whereas IL-4 was first detected on day 14. In contrast, only IFN-γ could be detected in C3H/HeN mice, and the levels steadily increased from day 2 to surpass those seen in BALB/c mice by day 14 of infection. Despite the difference in cytokine profiles, both BALB/c and C3H/HeN mice developed comparable arthritis assessed at 14 days of infection. Arthritis regressed by day 30 in BALB/c mice but persisted in C3H/HeN mice. These studies are the first to demonstrate that the Th2 response to Borrelia burgdorferi infection of BALB/c mice is preceded by a Th1 cytokine response. Moreover, the timing of the appearance of IL-4 suggests that its primary effect is not in preventing disease, as suggested by others, but, rather, in hastening the resolution of inflammation. The implications of these findings for the orchestration of host defense against B. burgdorferi infection are discussed.

Original languageEnglish (US)
Pages (from-to)3107-3111
Number of pages5
JournalInfection and Immunity
Volume65
Issue number8
StatePublished - 1997
Externally publishedYes

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Lyme Disease
Helper-Inducer T-Lymphocytes
Inbred C3H Mouse
Cytokines
Borrelia Infections
Interleukin-4
Borrelia burgdorferi
Infection
Interferons
Arthritis
Th2 Cells
Genetic Predisposition to Disease
Interferon-gamma
Lymph Nodes
Inflammation
T-Lymphocytes
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Kang, I., Barthold, S. W., Persing, D. H., & Bockenstedt, L. K. (1997). T-helper-cell cytokines in the early evolution of murine Lyme arthritis. Infection and Immunity, 65(8), 3107-3111.

T-helper-cell cytokines in the early evolution of murine Lyme arthritis. / Kang, Insoo; Barthold, Stephen W; Persing, David H.; Bockenstedt, Linda K.

In: Infection and Immunity, Vol. 65, No. 8, 1997, p. 3107-3111.

Research output: Contribution to journalArticle

Kang, I, Barthold, SW, Persing, DH & Bockenstedt, LK 1997, 'T-helper-cell cytokines in the early evolution of murine Lyme arthritis', Infection and Immunity, vol. 65, no. 8, pp. 3107-3111.
Kang, Insoo ; Barthold, Stephen W ; Persing, David H. ; Bockenstedt, Linda K. / T-helper-cell cytokines in the early evolution of murine Lyme arthritis. In: Infection and Immunity. 1997 ; Vol. 65, No. 8. pp. 3107-3111.
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