T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure

Angela Franciska Haczku, R. Moqbel, M. Jacobson, A. B. Kay, P. J. Barnes, K. F. Chung

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Abstract

We have investigated the relationship between changes in T-cell activation in the bronchial mucosa, airway responsiveness and eosinophilic inflammation in sensitized Brown-Norway rats exposed to ovalbumin (OVA). Rats sensitized intraperitoneally with OVA and exposed to OVA aerosol 21 days later showed an enhanced increase in lung resistance (RNL) to acetylcholine (P < 0.05), and a significant increase in the number of eosinophils, neutrophils and lymphocytes in bronchoalveolar lavage fluid (BAL) (P < 0.05), compared with sensitized but saline-exposed controls. There was a significant increase in cells expressing the T-cell activation marker CD25 (P < 0.05) and the numbers of CD8+ T cells (P < 0.05), but not in the numbers of CD2+ and CD4+ cells. Eosinophil counts in airway submucosal tissue, as assessed by staining with BMK-13; a monoclonal antibody that binds to eosinophil major basic protein (MBP), were increased in rats receiving sensitization and exposure to OVA compared with naive controls (P < 0.002). There were significant positive correlations between the increase in R(L) to acetylcholine and the numbers of CD25+ (r = 0.92, P < 0.001), CD4+ (r = 0.77, P < 0.05), CD8+ (r = 0.71, P < 0.05) and MBP+ (r = 0.72, P < 0.03) cells in the OVA-sensitized and exposed group, but not in saline-exposed or naive animals. The number of MBP+ cells also correlated with CD25 expression (r = 0.71, P < 0.05). We conclude that airway hyper-responsiveness and inflammatory cell infiltration caused by OVA exposure of sensitized animals is associated with the presence of activated T cells in the airway mucosa. CD8+ T cells may play a role in the regulation of events leading to eosinophil inflammation and airway hyper-responsiveness.

Original languageEnglish (US)
Pages (from-to)591-597
Number of pages7
JournalImmunology
Volume85
Issue number4
StatePublished - 1995
Externally publishedYes

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Ovalbumin
T-Lymphocyte Subsets
Allergens
Mucous Membrane
Respiratory Hypersensitivity
T-Lymphocytes
Eosinophils
Acetylcholine
Eosinophil Major Basic Protein
Inflammation
Bronchoalveolar Lavage Fluid
Aerosols
Proteins
Neutrophils
Monoclonal Antibodies
Lymphocytes
Staining and Labeling
Lung

ASJC Scopus subject areas

  • Immunology

Cite this

Haczku, A. F., Moqbel, R., Jacobson, M., Kay, A. B., Barnes, P. J., & Chung, K. F. (1995). T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure. Immunology, 85(4), 591-597.

T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure. / Haczku, Angela Franciska; Moqbel, R.; Jacobson, M.; Kay, A. B.; Barnes, P. J.; Chung, K. F.

In: Immunology, Vol. 85, No. 4, 1995, p. 591-597.

Research output: Contribution to journalArticle

Haczku, AF, Moqbel, R, Jacobson, M, Kay, AB, Barnes, PJ & Chung, KF 1995, 'T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure', Immunology, vol. 85, no. 4, pp. 591-597.
Haczku, Angela Franciska ; Moqbel, R. ; Jacobson, M. ; Kay, A. B. ; Barnes, P. J. ; Chung, K. F. / T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure. In: Immunology. 1995 ; Vol. 85, No. 4. pp. 591-597.
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AU - Haczku, Angela Franciska

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AU - Barnes, P. J.

AU - Chung, K. F.

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N2 - We have investigated the relationship between changes in T-cell activation in the bronchial mucosa, airway responsiveness and eosinophilic inflammation in sensitized Brown-Norway rats exposed to ovalbumin (OVA). Rats sensitized intraperitoneally with OVA and exposed to OVA aerosol 21 days later showed an enhanced increase in lung resistance (RNL) to acetylcholine (P < 0.05), and a significant increase in the number of eosinophils, neutrophils and lymphocytes in bronchoalveolar lavage fluid (BAL) (P < 0.05), compared with sensitized but saline-exposed controls. There was a significant increase in cells expressing the T-cell activation marker CD25 (P < 0.05) and the numbers of CD8+ T cells (P < 0.05), but not in the numbers of CD2+ and CD4+ cells. Eosinophil counts in airway submucosal tissue, as assessed by staining with BMK-13; a monoclonal antibody that binds to eosinophil major basic protein (MBP), were increased in rats receiving sensitization and exposure to OVA compared with naive controls (P < 0.002). There were significant positive correlations between the increase in R(L) to acetylcholine and the numbers of CD25+ (r = 0.92, P < 0.001), CD4+ (r = 0.77, P < 0.05), CD8+ (r = 0.71, P < 0.05) and MBP+ (r = 0.72, P < 0.03) cells in the OVA-sensitized and exposed group, but not in saline-exposed or naive animals. The number of MBP+ cells also correlated with CD25 expression (r = 0.71, P < 0.05). We conclude that airway hyper-responsiveness and inflammatory cell infiltration caused by OVA exposure of sensitized animals is associated with the presence of activated T cells in the airway mucosa. CD8+ T cells may play a role in the regulation of events leading to eosinophil inflammation and airway hyper-responsiveness.

AB - We have investigated the relationship between changes in T-cell activation in the bronchial mucosa, airway responsiveness and eosinophilic inflammation in sensitized Brown-Norway rats exposed to ovalbumin (OVA). Rats sensitized intraperitoneally with OVA and exposed to OVA aerosol 21 days later showed an enhanced increase in lung resistance (RNL) to acetylcholine (P < 0.05), and a significant increase in the number of eosinophils, neutrophils and lymphocytes in bronchoalveolar lavage fluid (BAL) (P < 0.05), compared with sensitized but saline-exposed controls. There was a significant increase in cells expressing the T-cell activation marker CD25 (P < 0.05) and the numbers of CD8+ T cells (P < 0.05), but not in the numbers of CD2+ and CD4+ cells. Eosinophil counts in airway submucosal tissue, as assessed by staining with BMK-13; a monoclonal antibody that binds to eosinophil major basic protein (MBP), were increased in rats receiving sensitization and exposure to OVA compared with naive controls (P < 0.002). There were significant positive correlations between the increase in R(L) to acetylcholine and the numbers of CD25+ (r = 0.92, P < 0.001), CD4+ (r = 0.77, P < 0.05), CD8+ (r = 0.71, P < 0.05) and MBP+ (r = 0.72, P < 0.03) cells in the OVA-sensitized and exposed group, but not in saline-exposed or naive animals. The number of MBP+ cells also correlated with CD25 expression (r = 0.71, P < 0.05). We conclude that airway hyper-responsiveness and inflammatory cell infiltration caused by OVA exposure of sensitized animals is associated with the presence of activated T cells in the airway mucosa. CD8+ T cells may play a role in the regulation of events leading to eosinophil inflammation and airway hyper-responsiveness.

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