T cells regulate Herpes Simplex virus replication in the trigeminal ganglion

R. L. Hendricks, P. Kodukula, Roger Sciammas, T. Liu, J. Bluestone, J. A. Bluestone

Research output: Contribution to journalArticle

Abstract

Purpose. Herpes Simplex virus type 1 (HSV-1) is transmitted to the trigeminal ganglion (TG) of mice within 2 days of corneal infection, where it replicates briefly (2-9 days post infection [p.i.]) and then establishes a latent infection. The purpose of these studies was to identify the T cell populations that contribute to control of HSV-1 replication in the TG. Methods. Normal Balb/c mice, or Balb/c mice with a disrupted T cell receptor (TCR) α gene (αβ TCR null) were studied untreated or when depleted of γδ TCR+ cells by injection of the monoclonal antibody (GL3). At various times after HSV-1 corneal infection, the TG were excised and the presence of HSV antigens, T cells, and tumor necrosis factor (TNF) was evaluated by immunoperoxidase staining. Results. Normal mice showed an early accumulation of γδ TCR+ cells (days 3-7 p.i.), and a later infiltration of CD8+ cells (days 5-12 p.i.). Most of the CD8+ cells appeared to produce TNF, a cytokine that inhibits HSV-1 replication.. Depletion of γδ TCR+ cells from Balb/c mice enhanced early HSV-1 replication in the TG, but the virus was controlled by day 12 p.i. The αβ TCR null mice failed to control HSV-1 replication and died by day 30 p.i. Further depletion of γδ TCR+ cells from these mice enhanced early HSV-1 replication in the TG, and hastened death (day 15 p.i.). Conclusions. γδ TCR+ cells contribute to the control of acute HSV-1 replication in the TG, whereas CD8+ αβ TCR+ cells are required for complete eradication of replicating virus from the TG, and survival.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Trigeminal Ganglion
Simplexvirus
Virus Replication
Human Herpesvirus 1
T-Cell Antigen Receptor
T-Lymphocytes
Infection
Tumor Necrosis Factor-alpha
Viruses
T-Cell Receptor Genes
Monoclonal Antibodies
Staining and Labeling
Cytokines
Antigens
Injections

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Hendricks, R. L., Kodukula, P., Sciammas, R., Liu, T., Bluestone, J., & Bluestone, J. A. (1996). T cells regulate Herpes Simplex virus replication in the trigeminal ganglion. Investigative Ophthalmology and Visual Science, 37(3).

T cells regulate Herpes Simplex virus replication in the trigeminal ganglion. / Hendricks, R. L.; Kodukula, P.; Sciammas, Roger; Liu, T.; Bluestone, J.; Bluestone, J. A.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Hendricks, RL, Kodukula, P, Sciammas, R, Liu, T, Bluestone, J & Bluestone, JA 1996, 'T cells regulate Herpes Simplex virus replication in the trigeminal ganglion', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Hendricks, R. L. ; Kodukula, P. ; Sciammas, Roger ; Liu, T. ; Bluestone, J. ; Bluestone, J. A. / T cells regulate Herpes Simplex virus replication in the trigeminal ganglion. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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