T-Cell Presentation of Antigen Requires Cell-to-Cell Contact for Proliferation and Anergy Induction: Differential MHC Requirements for Superantigen and Autoantigen

Janine M LaSalle, Frances Toneguzzo, Mark Saadeh, David E. Golan, Robert Taber, David A. Hafler

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

MHC class II+ human T-cell clones are able to simultaneously present and respond to peptide Ag and superantigen resulting in both proliferation and subsequent anergy. A major question remains as to whether a single T cell can present to itself or whether T-T cell interactions are required. We have employed a novel technique for inhibiting cell-to-cell contact that encapsulates individual T cells in agarose gel microdrops. Myelin basic protein-reactive individual CD4+ T-cell clones entrapped within these microdrops neither proliferated nor became anergized to either peptide Ag or Staphylococcal enterotoxin B (SEB), suggesting that cell-to-cell contact was required for T-cell presentation of Ag leading to proliferation and anergy. PMA treatment induced T-cell migration out of gel microdrops restoring cell-to-cell contact and resulting in proliferation and anergy after T-cell coculture with peptide or superantigen. However, analysis of [Ca+2]i release revealed differences in T-cell responses to SEB versus peptide Ag. The addition of SEB, but not peptide Ag, induced a calcium flux in solitary T cells. Additionally, αHLA-DR mAb blocked peptide but not SEB-induced proliferation and anergy induction. Thus, SEB generated an early signal in solitary T cells that may not be a result of self stimulation via MHC class II. However, subsequent cell-to-cell contact was required for proliferation and anergy induction by SEB. These results indicate that peptide Ag requires a MHC class II-dependent cell-to-cell interaction for calcium flux, proliferation, and anergy induction, whereas SEB requires a MHC class II independent cell-to-cell interaction for proliferation and anergy induction after a TCR-generated calcium flux.

Original languageEnglish (US)
Pages (from-to)649-657
Number of pages9
JournalJournal of Immunology
Volume151
Issue number2
StatePublished - Jul 15 1993
Externally publishedYes

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Superantigens
Autoantigens
Antigen Presentation
Cell Proliferation
T-Lymphocytes
Peptides
Cell Communication
Calcium
Clone Cells
Gels
Self Stimulation
Myelin Basic Protein
HLA-DR Antigens
Coculture Techniques
staphylococcal enterotoxin B
Sepharose
Cell Movement

ASJC Scopus subject areas

  • Immunology

Cite this

T-Cell Presentation of Antigen Requires Cell-to-Cell Contact for Proliferation and Anergy Induction : Differential MHC Requirements for Superantigen and Autoantigen. / LaSalle, Janine M; Toneguzzo, Frances; Saadeh, Mark; Golan, David E.; Taber, Robert; Hafler, David A.

In: Journal of Immunology, Vol. 151, No. 2, 15.07.1993, p. 649-657.

Research output: Contribution to journalArticle

LaSalle, Janine M ; Toneguzzo, Frances ; Saadeh, Mark ; Golan, David E. ; Taber, Robert ; Hafler, David A. / T-Cell Presentation of Antigen Requires Cell-to-Cell Contact for Proliferation and Anergy Induction : Differential MHC Requirements for Superantigen and Autoantigen. In: Journal of Immunology. 1993 ; Vol. 151, No. 2. pp. 649-657.
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