T cell populations and cytokine expression in milk derived from normal and bacteria-infected bovine mammary glands

Bernadette C. Taylor, Robert G. Keefe, Jon D. Dellinger, Yoshihiro Nakamura, James S Cullor, Jeffrey L Stott

Research output: Contribution to journalArticle

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Abstract

This study investigated the effect of naturally acquired bacterial infection of the bovine mammary gland on subpopulations of T lymphocytes and cytokine expression in milk. Twenty-nine lactating cows with mastitis were compared to 12 normal animals. CD4+ lymphocytes represented a significantly greater percentage of the milk-derived lymphocytes in infected mammary glands compared to normal controls. Cytokine mRNA expression by cells derived from milk was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR). No IL-2 or IL-4 mRNA was detected in any samples, while IFN-γ mRNA was detected in all milk samples. IL-10 mRNA was detected in cells from the milk of 2 mastitic cows and 1 normal cow, and IL-12 mRNA was detected in 2 cows with mastitis. While TNF-α mRNA was not detected in this study, IL-6 mRNA was identified in cells from the milk of all animals, with levels being greater in mastitic animals.

Original languageEnglish (US)
Pages (from-to)68-76
Number of pages9
JournalCellular Immunology
Volume181
Issue number3
StatePublished - Nov 25 1997

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Human Mammary Glands
Milk
Cytokines
Bacteria
T-Lymphocytes
Messenger RNA
Population
Mastitis
Lymphocytes
Interleukin-12
Reverse Transcriptase Polymerase Chain Reaction
Bacterial Infections
Interleukin-4
Interleukin-10
Interleukin-2
Interleukin-6

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

T cell populations and cytokine expression in milk derived from normal and bacteria-infected bovine mammary glands. / Taylor, Bernadette C.; Keefe, Robert G.; Dellinger, Jon D.; Nakamura, Yoshihiro; Cullor, James S; Stott, Jeffrey L.

In: Cellular Immunology, Vol. 181, No. 3, 25.11.1997, p. 68-76.

Research output: Contribution to journalArticle

Taylor, Bernadette C. ; Keefe, Robert G. ; Dellinger, Jon D. ; Nakamura, Yoshihiro ; Cullor, James S ; Stott, Jeffrey L. / T cell populations and cytokine expression in milk derived from normal and bacteria-infected bovine mammary glands. In: Cellular Immunology. 1997 ; Vol. 181, No. 3. pp. 68-76.
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