T-Cell Epitope Immunotherapy in Mouse Models of Food Allergy

Christine Y.Y. Wai, Nicki Y.H. Leung, Ka Hou Chu, Patrick S.C. Leung

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Food allergy has been rising in prevalence over the last two decades, affecting more than 10% of the world population. Current management of IgE-mediated food allergy relies on avoidance and rescue medications; research into treatments that are safer and providing guaranteed and durable curative effects is, therefore, essential. T-cell epitope-based immunotherapy holds the potential for modulating food allergic responses without IgE cross-linking. In this chapter, we describe the methods in evaluating the therapeutic capacities of immunodominant T-cell epitopes in animal models of food allergy. Moreover, we explain in detail the methods to measure the allergen-specific antibody levels, prepare single-cell suspension from spleen, and prepare small intestine for immunohistochemical analysis of eosinophils and Foxp3+ cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages337-355
Number of pages19
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2223
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cow’s milk allergy
  • Egg allergy
  • ELISA
  • Eosinophils
  • Immunohistochemical staining (IHC)
  • Mouse model
  • Oral immunotherapy (OIT)
  • Regulatory T cell (Treg)
  • Shellfish allergy
  • Swiss roll

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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