@inbook{ba1205d9572a445a9377a7b407625397,
title = "T-Cell Epitope Immunotherapy in Mouse Models of Food Allergy",
abstract = "Food allergy has been rising in prevalence over the last two decades, affecting more than 10% of the world population. Current management of IgE-mediated food allergy relies on avoidance and rescue medications; research into treatments that are safer and providing guaranteed and durable curative effects is, therefore, essential. T-cell epitope-based immunotherapy holds the potential for modulating food allergic responses without IgE cross-linking. In this chapter, we describe the methods in evaluating the therapeutic capacities of immunodominant T-cell epitopes in animal models of food allergy. Moreover, we explain in detail the methods to measure the allergen-specific antibody levels, prepare single-cell suspension from spleen, and prepare small intestine for immunohistochemical analysis of eosinophils and Foxp3+ cells.",
keywords = "Cow{\textquoteright}s milk allergy, Egg allergy, ELISA, Eosinophils, Immunohistochemical staining (IHC), Mouse model, Oral immunotherapy (OIT), Regulatory T cell (Treg), Shellfish allergy, Swiss roll",
author = "Wai, {Christine Y.Y.} and Leung, {Nicki Y.H.} and Chu, {Ka Hou} and Leung, {Patrick S.C.}",
note = "Funding Information: C.Y.Y. Wai was supported by an AXA Postdoctoral Fellowship.",
year = "2021",
doi = "10.1007/978-1-0716-1001-5_21",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "337--355",
booktitle = "Methods in Molecular Biology",
}