T cell-dependent production of IFN-γ by NK cells in response to influenza a virus

Xiaosong He, Monia Draghi, Kutubuddin Mahmood, Tyson H. Holmes, George W. Kemble, Cornelia L. Dekker, Ann M. Arvin, Peter Parham, Harry B. Greenberg

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The role of human NK cells in viral infections is poorly understood. We used a cytokine flow-cytometry assay to simultaneously investigate the IFN-γ response of NK and T lymphocytes to influenza A virus (fluA). When PBMCs from fluA-immune adult donors were incubated with fluA, IFN-γ was produced by both CD56dim and CD56bright subsets of NK cells, as well as by fluA-specific T cells. Purified NK cells did not produce IFN-γ in response to fluA, while depletion of T lymphocytes reduced to background levels the fluA-induced IFN-γ production by NK cells, which indicates that T cells are required for the IFN-γ response of NK cells. The fluA-induced IFN-γ production of NK cells was suppressed by anti-IL-2 Ab, while recombinant IL-2 replaced the helper function of T cells for IFN-γ production by NK cells. This indicates that IL-2 produced by fluA-specific T cells is involved in the T cell-dependent IFN-γ response of NK cells to fluA. Taken together, these results suggest that at an early stage of recurrent viral infection, NK-mediated innate immunity to the virus is enhanced by preexisting virus-specific T cells.

Original languageEnglish (US)
Pages (from-to)1812-1819
Number of pages8
JournalJournal of Clinical Investigation
Volume114
Issue number12
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

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Orthomyxoviridae
Natural Killer Cells
T-Lymphocytes
Interleukin-2
Virus Diseases
Viruses
Influenza A virus
Helper-Inducer T-Lymphocytes
Innate Immunity
Flow Cytometry
Tissue Donors
Cytokines

ASJC Scopus subject areas

  • Medicine(all)

Cite this

He, X., Draghi, M., Mahmood, K., Holmes, T. H., Kemble, G. W., Dekker, C. L., ... Greenberg, H. B. (2004). T cell-dependent production of IFN-γ by NK cells in response to influenza a virus. Journal of Clinical Investigation, 114(12), 1812-1819. https://doi.org/10.1172/JCI22797

T cell-dependent production of IFN-γ by NK cells in response to influenza a virus. / He, Xiaosong; Draghi, Monia; Mahmood, Kutubuddin; Holmes, Tyson H.; Kemble, George W.; Dekker, Cornelia L.; Arvin, Ann M.; Parham, Peter; Greenberg, Harry B.

In: Journal of Clinical Investigation, Vol. 114, No. 12, 01.01.2004, p. 1812-1819.

Research output: Contribution to journalArticle

He, X, Draghi, M, Mahmood, K, Holmes, TH, Kemble, GW, Dekker, CL, Arvin, AM, Parham, P & Greenberg, HB 2004, 'T cell-dependent production of IFN-γ by NK cells in response to influenza a virus', Journal of Clinical Investigation, vol. 114, no. 12, pp. 1812-1819. https://doi.org/10.1172/JCI22797
He X, Draghi M, Mahmood K, Holmes TH, Kemble GW, Dekker CL et al. T cell-dependent production of IFN-γ by NK cells in response to influenza a virus. Journal of Clinical Investigation. 2004 Jan 1;114(12):1812-1819. https://doi.org/10.1172/JCI22797
He, Xiaosong ; Draghi, Monia ; Mahmood, Kutubuddin ; Holmes, Tyson H. ; Kemble, George W. ; Dekker, Cornelia L. ; Arvin, Ann M. ; Parham, Peter ; Greenberg, Harry B. / T cell-dependent production of IFN-γ by NK cells in response to influenza a virus. In: Journal of Clinical Investigation. 2004 ; Vol. 114, No. 12. pp. 1812-1819.
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