Abstract
The role of human NK cells in viral infections is poorly understood. We used a cytokine flow-cytometry assay to simultaneously investigate the IFN-γ response of NK and T lymphocytes to influenza A virus (fluA). When PBMCs from fluA-immune adult donors were incubated with fluA, IFN-γ was produced by both CD56dim and CD56bright subsets of NK cells, as well as by fluA-specific T cells. Purified NK cells did not produce IFN-γ in response to fluA, while depletion of T lymphocytes reduced to background levels the fluA-induced IFN-γ production by NK cells, which indicates that T cells are required for the IFN-γ response of NK cells. The fluA-induced IFN-γ production of NK cells was suppressed by anti-IL-2 Ab, while recombinant IL-2 replaced the helper function of T cells for IFN-γ production by NK cells. This indicates that IL-2 produced by fluA-specific T cells is involved in the T cell-dependent IFN-γ response of NK cells to fluA. Taken together, these results suggest that at an early stage of recurrent viral infection, NK-mediated innate immunity to the virus is enhanced by preexisting virus-specific T cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1812-1819 |
| Number of pages | 8 |
| Journal | Journal of Clinical Investigation |
| Volume | 114 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jan 1 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
T cell-dependent production of IFN-γ by NK cells in response to influenza a virus. / He, Xiaosong; Draghi, Monia; Mahmood, Kutubuddin; Holmes, Tyson H.; Kemble, George W.; Dekker, Cornelia L.; Arvin, Ann M.; Parham, Peter; Greenberg, Harry B.
In: Journal of Clinical Investigation, Vol. 114, No. 12, 01.01.2004, p. 1812-1819.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - T cell-dependent production of IFN-γ by NK cells in response to influenza a virus
AU - He, Xiaosong
AU - Draghi, Monia
AU - Mahmood, Kutubuddin
AU - Holmes, Tyson H.
AU - Kemble, George W.
AU - Dekker, Cornelia L.
AU - Arvin, Ann M.
AU - Parham, Peter
AU - Greenberg, Harry B.
PY - 2004/1/1
Y1 - 2004/1/1
N2 - The role of human NK cells in viral infections is poorly understood. We used a cytokine flow-cytometry assay to simultaneously investigate the IFN-γ response of NK and T lymphocytes to influenza A virus (fluA). When PBMCs from fluA-immune adult donors were incubated with fluA, IFN-γ was produced by both CD56dim and CD56bright subsets of NK cells, as well as by fluA-specific T cells. Purified NK cells did not produce IFN-γ in response to fluA, while depletion of T lymphocytes reduced to background levels the fluA-induced IFN-γ production by NK cells, which indicates that T cells are required for the IFN-γ response of NK cells. The fluA-induced IFN-γ production of NK cells was suppressed by anti-IL-2 Ab, while recombinant IL-2 replaced the helper function of T cells for IFN-γ production by NK cells. This indicates that IL-2 produced by fluA-specific T cells is involved in the T cell-dependent IFN-γ response of NK cells to fluA. Taken together, these results suggest that at an early stage of recurrent viral infection, NK-mediated innate immunity to the virus is enhanced by preexisting virus-specific T cells.
AB - The role of human NK cells in viral infections is poorly understood. We used a cytokine flow-cytometry assay to simultaneously investigate the IFN-γ response of NK and T lymphocytes to influenza A virus (fluA). When PBMCs from fluA-immune adult donors were incubated with fluA, IFN-γ was produced by both CD56dim and CD56bright subsets of NK cells, as well as by fluA-specific T cells. Purified NK cells did not produce IFN-γ in response to fluA, while depletion of T lymphocytes reduced to background levels the fluA-induced IFN-γ production by NK cells, which indicates that T cells are required for the IFN-γ response of NK cells. The fluA-induced IFN-γ production of NK cells was suppressed by anti-IL-2 Ab, while recombinant IL-2 replaced the helper function of T cells for IFN-γ production by NK cells. This indicates that IL-2 produced by fluA-specific T cells is involved in the T cell-dependent IFN-γ response of NK cells to fluA. Taken together, these results suggest that at an early stage of recurrent viral infection, NK-mediated innate immunity to the virus is enhanced by preexisting virus-specific T cells.
UR - http://www.scopus.com/inward/record.url?scp=85047689873&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047689873&partnerID=8YFLogxK
U2 - 10.1172/JCI22797
DO - 10.1172/JCI22797
M3 - Article
C2 - 15599406
AN - SCOPUS:85047689873
VL - 114
SP - 1812
EP - 1819
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 12
ER -