T cell and APC dynamics in situ control the outcome of vaccination

Kamal M. Khanna, David A. Blair, Anthony T. Vella, Stephen J Mcsorley, Sandip K. Datta, Leo Lefrançois

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The factors controlling the progression of an immune response to generation of protective memory are poorly understood. We compared the in situ and ex vivo characteristics of CD8 T cells responding to different forms of the same immunogen. Immunization with live Listeria monocytogenes, irradiated L. monocytogenes (IRL), or heat-killed L. monocytogenes (HKL) induced rapid activation of CD8 T cells. However, only IRL and live L. monocytogenes inoculation induced sustained proliferation and supported memory development. Gene and protein expression analysis revealed that the three forms of immunization led to three distinct transcriptional and translational programs. Prior to cell division, CD8 T cell-dendritic cell clusters formed in the spleen after live L. monocytogenes and IRL but not after HKL immunization. Furthermore, HKL immunization induced rapid remodeling of splenic architecture, including loss of marginal zone macrophages, which resulted in impaired bacterial clearance. These results identify initial characteristics of a protective T cell response that have implications for the development of more effective vaccination strategies.

Original languageEnglish (US)
Pages (from-to)239-252
Number of pages14
JournalJournal of Immunology
Volume185
Issue number1
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

Fingerprint

Listeria monocytogenes
Vaccination
T-Lymphocytes
Immunization
Hot Temperature
Cell Division
Dendritic Cells
Spleen
Macrophages
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Khanna, K. M., Blair, D. A., Vella, A. T., Mcsorley, S. J., Datta, S. K., & Lefrançois, L. (2010). T cell and APC dynamics in situ control the outcome of vaccination. Journal of Immunology, 185(1), 239-252. https://doi.org/10.4049/jimmunol.0901047

T cell and APC dynamics in situ control the outcome of vaccination. / Khanna, Kamal M.; Blair, David A.; Vella, Anthony T.; Mcsorley, Stephen J; Datta, Sandip K.; Lefrançois, Leo.

In: Journal of Immunology, Vol. 185, No. 1, 01.07.2010, p. 239-252.

Research output: Contribution to journalArticle

Khanna, KM, Blair, DA, Vella, AT, Mcsorley, SJ, Datta, SK & Lefrançois, L 2010, 'T cell and APC dynamics in situ control the outcome of vaccination', Journal of Immunology, vol. 185, no. 1, pp. 239-252. https://doi.org/10.4049/jimmunol.0901047
Khanna, Kamal M. ; Blair, David A. ; Vella, Anthony T. ; Mcsorley, Stephen J ; Datta, Sandip K. ; Lefrançois, Leo. / T cell and APC dynamics in situ control the outcome of vaccination. In: Journal of Immunology. 2010 ; Vol. 185, No. 1. pp. 239-252.
@article{099d59c01cc1412dbb18691a301d2b5f,
title = "T cell and APC dynamics in situ control the outcome of vaccination",
abstract = "The factors controlling the progression of an immune response to generation of protective memory are poorly understood. We compared the in situ and ex vivo characteristics of CD8 T cells responding to different forms of the same immunogen. Immunization with live Listeria monocytogenes, irradiated L. monocytogenes (IRL), or heat-killed L. monocytogenes (HKL) induced rapid activation of CD8 T cells. However, only IRL and live L. monocytogenes inoculation induced sustained proliferation and supported memory development. Gene and protein expression analysis revealed that the three forms of immunization led to three distinct transcriptional and translational programs. Prior to cell division, CD8 T cell-dendritic cell clusters formed in the spleen after live L. monocytogenes and IRL but not after HKL immunization. Furthermore, HKL immunization induced rapid remodeling of splenic architecture, including loss of marginal zone macrophages, which resulted in impaired bacterial clearance. These results identify initial characteristics of a protective T cell response that have implications for the development of more effective vaccination strategies.",
author = "Khanna, {Kamal M.} and Blair, {David A.} and Vella, {Anthony T.} and Mcsorley, {Stephen J} and Datta, {Sandip K.} and Leo Lefran{\cc}ois",
year = "2010",
month = "7",
day = "1",
doi = "10.4049/jimmunol.0901047",
language = "English (US)",
volume = "185",
pages = "239--252",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

TY - JOUR

T1 - T cell and APC dynamics in situ control the outcome of vaccination

AU - Khanna, Kamal M.

AU - Blair, David A.

AU - Vella, Anthony T.

AU - Mcsorley, Stephen J

AU - Datta, Sandip K.

AU - Lefrançois, Leo

PY - 2010/7/1

Y1 - 2010/7/1

N2 - The factors controlling the progression of an immune response to generation of protective memory are poorly understood. We compared the in situ and ex vivo characteristics of CD8 T cells responding to different forms of the same immunogen. Immunization with live Listeria monocytogenes, irradiated L. monocytogenes (IRL), or heat-killed L. monocytogenes (HKL) induced rapid activation of CD8 T cells. However, only IRL and live L. monocytogenes inoculation induced sustained proliferation and supported memory development. Gene and protein expression analysis revealed that the three forms of immunization led to three distinct transcriptional and translational programs. Prior to cell division, CD8 T cell-dendritic cell clusters formed in the spleen after live L. monocytogenes and IRL but not after HKL immunization. Furthermore, HKL immunization induced rapid remodeling of splenic architecture, including loss of marginal zone macrophages, which resulted in impaired bacterial clearance. These results identify initial characteristics of a protective T cell response that have implications for the development of more effective vaccination strategies.

AB - The factors controlling the progression of an immune response to generation of protective memory are poorly understood. We compared the in situ and ex vivo characteristics of CD8 T cells responding to different forms of the same immunogen. Immunization with live Listeria monocytogenes, irradiated L. monocytogenes (IRL), or heat-killed L. monocytogenes (HKL) induced rapid activation of CD8 T cells. However, only IRL and live L. monocytogenes inoculation induced sustained proliferation and supported memory development. Gene and protein expression analysis revealed that the three forms of immunization led to three distinct transcriptional and translational programs. Prior to cell division, CD8 T cell-dendritic cell clusters formed in the spleen after live L. monocytogenes and IRL but not after HKL immunization. Furthermore, HKL immunization induced rapid remodeling of splenic architecture, including loss of marginal zone macrophages, which resulted in impaired bacterial clearance. These results identify initial characteristics of a protective T cell response that have implications for the development of more effective vaccination strategies.

UR - http://www.scopus.com/inward/record.url?scp=77956202725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956202725&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.0901047

DO - 10.4049/jimmunol.0901047

M3 - Article

C2 - 20530268

AN - SCOPUS:77956202725

VL - 185

SP - 239

EP - 252

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -