T-bet is a STATI-induced regulator for IL-12R expression in naïve CD4+ T cells

Maryam Afkarian, John R. Sedy, Jianfei Yang, Nils G. Jacobson, Nezih Cereb, Soo Y. Yang, Theresa L. Murphy, Kenneth M. Murphy

Research output: Contribution to journalArticle

695 Scopus citations

Abstract

T helper type I (TH1) cell development involves interferon-γ (IFN-γ) signaling through signal transducer and activator of transcription I (STAT I) and interleukin-12 (IL-12) signaling through STAT4 activation. We examined here T-bet regulation and evaluated the actions of T-bet in STAT1- and STAT4-dependent TH1 development processes. We found that T-bet expression during T cell activation was strongly dependent on IFN-γ signaling and STATI activation, but was independent of STAT4. Ectopic T-bet expression strongly increased IFN-γ production in TH2 cells activated by PMA-ionomycin, but weakly increased IFN-γ production in TH2 cells stimulated by IL-12-IL-18 or OVA peptide-antigen-presenting cell stimulation. In contrast, IL-12-IL-18-induced IFN-γ production remained STAT4-dependent despite ectopic T-bet expression. Ectopic T-bet expression selectively induced expression of IL-12Rβ2, but not IL-18Rα, in wild-type and STAT1−/− TH2 cells, but did not extinguish expression of GATA-3 and TH2 cytokines. Finally, ectopic T-bet did not directly induce expression of endogenous T- bet independently of IFN-γ or STAT1. Thus, T-bet is induced by IFN-γ and STAT1 signaling during T cell activation. In addition, T-bet mediates STAT1-dependent processes of TH1 development, including the induction of IL-12Rβ2.

Original languageEnglish (US)
Pages (from-to)549-557
Number of pages9
JournalNature Immunology
Volume3
Issue number6
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Afkarian, M., Sedy, J. R., Yang, J., Jacobson, N. G., Cereb, N., Yang, S. Y., Murphy, T. L., & Murphy, K. M. (2002). T-bet is a STATI-induced regulator for IL-12R expression in naïve CD4+ T cells. Nature Immunology, 3(6), 549-557. https://doi.org/10.1038/ni794