Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens

Abhishek Santra, Hai Yu, Nova Tasnima, Musleh M. Muthana, Yanhong Li, Jie Zeng, Nicholas Kenyon, Angelique Y. Louie, Xi Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes. Taking advantage of a bacterial sialyltransferase mutant that can catalyze the transfer of different sialic acid forms from the corresponding sugar nucleotide donors to Lewis x antigens, which are fucosylated glycans, as well as an efficient one-pot multienzyme (OPME) sialylation system, O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations were systematically synthesized by chemoenzymatic methods.

Original languageEnglish (US)
Pages (from-to)2827-2831
Number of pages5
JournalChemical Science
Volume7
Issue number4
DOIs
StatePublished - Apr 1 2016

Fingerprint

N-Acetylneuraminic Acid
Sialyltransferases
Sugars
Polysaccharides
Nucleotides
Antigens
sialyl Lewis X antigen

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Santra, A., Yu, H., Tasnima, N., Muthana, M. M., Li, Y., Zeng, J., ... Chen, X. (2016). Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens. Chemical Science, 7(4), 2827-2831. https://doi.org/10.1039/c5sc04104j

Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens. / Santra, Abhishek; Yu, Hai; Tasnima, Nova; Muthana, Musleh M.; Li, Yanhong; Zeng, Jie; Kenyon, Nicholas; Louie, Angelique Y.; Chen, Xi.

In: Chemical Science, Vol. 7, No. 4, 01.04.2016, p. 2827-2831.

Research output: Contribution to journalArticle

Santra, A, Yu, H, Tasnima, N, Muthana, MM, Li, Y, Zeng, J, Kenyon, N, Louie, AY & Chen, X 2016, 'Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens', Chemical Science, vol. 7, no. 4, pp. 2827-2831. https://doi.org/10.1039/c5sc04104j
Santra A, Yu H, Tasnima N, Muthana MM, Li Y, Zeng J et al. Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens. Chemical Science. 2016 Apr 1;7(4):2827-2831. https://doi.org/10.1039/c5sc04104j
Santra, Abhishek ; Yu, Hai ; Tasnima, Nova ; Muthana, Musleh M. ; Li, Yanhong ; Zeng, Jie ; Kenyon, Nicholas ; Louie, Angelique Y. ; Chen, Xi. / Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens. In: Chemical Science. 2016 ; Vol. 7, No. 4. pp. 2827-2831.
@article{07a5cc80deed4311b12d15dec4aaf05a,
title = "Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens",
abstract = "O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes. Taking advantage of a bacterial sialyltransferase mutant that can catalyze the transfer of different sialic acid forms from the corresponding sugar nucleotide donors to Lewis x antigens, which are fucosylated glycans, as well as an efficient one-pot multienzyme (OPME) sialylation system, O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations were systematically synthesized by chemoenzymatic methods.",
author = "Abhishek Santra and Hai Yu and Nova Tasnima and Muthana, {Musleh M.} and Yanhong Li and Jie Zeng and Nicholas Kenyon and Louie, {Angelique Y.} and Xi Chen",
year = "2016",
month = "4",
day = "1",
doi = "10.1039/c5sc04104j",
language = "English (US)",
volume = "7",
pages = "2827--2831",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "4",

}

TY - JOUR

T1 - Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens

AU - Santra, Abhishek

AU - Yu, Hai

AU - Tasnima, Nova

AU - Muthana, Musleh M.

AU - Li, Yanhong

AU - Zeng, Jie

AU - Kenyon, Nicholas

AU - Louie, Angelique Y.

AU - Chen, Xi

PY - 2016/4/1

Y1 - 2016/4/1

N2 - O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes. Taking advantage of a bacterial sialyltransferase mutant that can catalyze the transfer of different sialic acid forms from the corresponding sugar nucleotide donors to Lewis x antigens, which are fucosylated glycans, as well as an efficient one-pot multienzyme (OPME) sialylation system, O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations were systematically synthesized by chemoenzymatic methods.

AB - O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes. Taking advantage of a bacterial sialyltransferase mutant that can catalyze the transfer of different sialic acid forms from the corresponding sugar nucleotide donors to Lewis x antigens, which are fucosylated glycans, as well as an efficient one-pot multienzyme (OPME) sialylation system, O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations were systematically synthesized by chemoenzymatic methods.

UR - http://www.scopus.com/inward/record.url?scp=84962324033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962324033&partnerID=8YFLogxK

U2 - 10.1039/c5sc04104j

DO - 10.1039/c5sc04104j

M3 - Article

AN - SCOPUS:84962324033

VL - 7

SP - 2827

EP - 2831

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

IS - 4

ER -