System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium

Do Yup Lee, Jeong Jin Park, Dinesh K. Barupal, Oliver Fiehn

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Drastic alterations in macronutrients are known to cause large changes in biochemistry and gene expression in the photosynthetic alga Chlamydomonas reinhardtii. However, metabolomic and proteomic responses to subtle reductions in macronutrients have not yet been studied. When ammonium levels were reduced by 25-100% compared with control cultures, ammonium uptake and growth rates were not affected at 25% or 50% nitrogen-reduction for 28 h. However, primary metabolism and enzyme expression showed remarkable changes at acute conditions (4 h and 10 h after ammonium reduction) compared with chronic conditions (18 h and 28 h time points). Responses of 145 identified metabolites were quantified using gas chromatography-time of flight mass spectrometry; 495 proteins (including 187 enzymes) were monitored using liquid chromatography-ion trap mass spectrometry with label-free spectral counting. Stress response and carbon assimilation processes (Calvin cycle, acetate uptake and chlorophyll biosynthesis) were altered first, in addition to increase in enzyme contents for lipid biosynthesis and accumulation of short chain free fatty acids. Nitrogen/carbon balance metabolism was found changed only under chronic conditions, for example in the citric acid cycle and amino acid metabolism. Metabolism in Chlamydomonas readily responds to total available media nitrogen with temporal increases in short-chain free fatty acids and turnover of internal proteins, long before nitrogen resources are depleted.

Original languageEnglish (US)
Pages (from-to)973-988
Number of pages16
JournalMolecular and Cellular Proteomics
Volume11
Issue number10
DOIs
StatePublished - Oct 2012

Fingerprint

Chlamydomonas reinhardtii
Metabolic Networks and Pathways
Ammonium Compounds
Metabolism
Nitrogen
Volatile Fatty Acids
Biosynthesis
Nonesterified Fatty Acids
Mass spectrometry
Mass Spectrometry
Enzymes
Carbon
Chlamydomonas
Biochemistry
Metabolomics
Citric Acid Cycle
Liquid chromatography
Photosynthesis
Chlorophyll
Algae

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium. / Lee, Do Yup; Park, Jeong Jin; Barupal, Dinesh K.; Fiehn, Oliver.

In: Molecular and Cellular Proteomics, Vol. 11, No. 10, 10.2012, p. 973-988.

Research output: Contribution to journalArticle

Lee, Do Yup ; Park, Jeong Jin ; Barupal, Dinesh K. ; Fiehn, Oliver. / System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium. In: Molecular and Cellular Proteomics. 2012 ; Vol. 11, No. 10. pp. 973-988.
@article{0738a2161b5142f79661060d8292c1a4,
title = "System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium",
abstract = "Drastic alterations in macronutrients are known to cause large changes in biochemistry and gene expression in the photosynthetic alga Chlamydomonas reinhardtii. However, metabolomic and proteomic responses to subtle reductions in macronutrients have not yet been studied. When ammonium levels were reduced by 25-100{\%} compared with control cultures, ammonium uptake and growth rates were not affected at 25{\%} or 50{\%} nitrogen-reduction for 28 h. However, primary metabolism and enzyme expression showed remarkable changes at acute conditions (4 h and 10 h after ammonium reduction) compared with chronic conditions (18 h and 28 h time points). Responses of 145 identified metabolites were quantified using gas chromatography-time of flight mass spectrometry; 495 proteins (including 187 enzymes) were monitored using liquid chromatography-ion trap mass spectrometry with label-free spectral counting. Stress response and carbon assimilation processes (Calvin cycle, acetate uptake and chlorophyll biosynthesis) were altered first, in addition to increase in enzyme contents for lipid biosynthesis and accumulation of short chain free fatty acids. Nitrogen/carbon balance metabolism was found changed only under chronic conditions, for example in the citric acid cycle and amino acid metabolism. Metabolism in Chlamydomonas readily responds to total available media nitrogen with temporal increases in short-chain free fatty acids and turnover of internal proteins, long before nitrogen resources are depleted.",
author = "Lee, {Do Yup} and Park, {Jeong Jin} and Barupal, {Dinesh K.} and Oliver Fiehn",
year = "2012",
month = "10",
doi = "10.1074/mcp.M111.016733",
language = "English (US)",
volume = "11",
pages = "973--988",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "10",

}

TY - JOUR

T1 - System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium

AU - Lee, Do Yup

AU - Park, Jeong Jin

AU - Barupal, Dinesh K.

AU - Fiehn, Oliver

PY - 2012/10

Y1 - 2012/10

N2 - Drastic alterations in macronutrients are known to cause large changes in biochemistry and gene expression in the photosynthetic alga Chlamydomonas reinhardtii. However, metabolomic and proteomic responses to subtle reductions in macronutrients have not yet been studied. When ammonium levels were reduced by 25-100% compared with control cultures, ammonium uptake and growth rates were not affected at 25% or 50% nitrogen-reduction for 28 h. However, primary metabolism and enzyme expression showed remarkable changes at acute conditions (4 h and 10 h after ammonium reduction) compared with chronic conditions (18 h and 28 h time points). Responses of 145 identified metabolites were quantified using gas chromatography-time of flight mass spectrometry; 495 proteins (including 187 enzymes) were monitored using liquid chromatography-ion trap mass spectrometry with label-free spectral counting. Stress response and carbon assimilation processes (Calvin cycle, acetate uptake and chlorophyll biosynthesis) were altered first, in addition to increase in enzyme contents for lipid biosynthesis and accumulation of short chain free fatty acids. Nitrogen/carbon balance metabolism was found changed only under chronic conditions, for example in the citric acid cycle and amino acid metabolism. Metabolism in Chlamydomonas readily responds to total available media nitrogen with temporal increases in short-chain free fatty acids and turnover of internal proteins, long before nitrogen resources are depleted.

AB - Drastic alterations in macronutrients are known to cause large changes in biochemistry and gene expression in the photosynthetic alga Chlamydomonas reinhardtii. However, metabolomic and proteomic responses to subtle reductions in macronutrients have not yet been studied. When ammonium levels were reduced by 25-100% compared with control cultures, ammonium uptake and growth rates were not affected at 25% or 50% nitrogen-reduction for 28 h. However, primary metabolism and enzyme expression showed remarkable changes at acute conditions (4 h and 10 h after ammonium reduction) compared with chronic conditions (18 h and 28 h time points). Responses of 145 identified metabolites were quantified using gas chromatography-time of flight mass spectrometry; 495 proteins (including 187 enzymes) were monitored using liquid chromatography-ion trap mass spectrometry with label-free spectral counting. Stress response and carbon assimilation processes (Calvin cycle, acetate uptake and chlorophyll biosynthesis) were altered first, in addition to increase in enzyme contents for lipid biosynthesis and accumulation of short chain free fatty acids. Nitrogen/carbon balance metabolism was found changed only under chronic conditions, for example in the citric acid cycle and amino acid metabolism. Metabolism in Chlamydomonas readily responds to total available media nitrogen with temporal increases in short-chain free fatty acids and turnover of internal proteins, long before nitrogen resources are depleted.

UR - http://www.scopus.com/inward/record.url?scp=84867161579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867161579&partnerID=8YFLogxK

U2 - 10.1074/mcp.M111.016733

DO - 10.1074/mcp.M111.016733

M3 - Article

C2 - 22787274

AN - SCOPUS:84867161579

VL - 11

SP - 973

EP - 988

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

IS - 10

ER -