Synthetic substrate analogs for the RNA-editing adenosine deaminase ADAR-2

Hye Young Yi-Brunozzi, LaHoma M. Easterwood, Gregg M. Kamilar, Peter A. Beal

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43 Scopus citations

Abstract

We have synthesized structural analogs of a natural RNA editing substrate and compared editing reactions of these substrates by recombinant ADAR-2, an RNA-editing adenosine deaminase. Deamination rates were shown to be sensitive to structural changes at the 2'-carbon of the edited adenosine. Methylation of the 2'-OH caused a large decrease in deamination rate, whereas 2'-deoxyadenosine and 2'-deoxy-2'-fluoroadenosine were deaminated at a rate similar to adenosine. In addition, a duplex containing as few as 19 bp of the stem structure adjacent to the R/G editing site of the GluR-B pre-mRNA supports deamination of the R/G adenosine by ADAR-2. This identification and initial characterization of synthetic RNA editing substrate analogs further defines structural elements in the RNA that are important for the deamination reaction and sets the stage for additional detailed structural, thermodynamic and kinetic studies of the ADAR-2 reaction.

Original languageEnglish (US)
Pages (from-to)2912-2917
Number of pages6
JournalNucleic Acids Research
Volume27
Issue number14
DOIs
StatePublished - Jul 15 1999
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Yi-Brunozzi, H. Y., Easterwood, L. M., Kamilar, G. M., & Beal, P. A. (1999). Synthetic substrate analogs for the RNA-editing adenosine deaminase ADAR-2. Nucleic Acids Research, 27(14), 2912-2917. https://doi.org/10.1093/nar/27.14.2912