Synthetic blends of volatile, phytopathogen-induced odorants can be used to manipulate vector behavior

Alexander A. Aksenov, Xavier Martini, Weixiang Zhao, Lukasz L. Stelinski, Cristina E Davis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Volatile organic compounds (VOCs) are emitted from all plants and these VOCs are important means of communication between plants and insects. It has been documented that pathogen infections alter VOC profiles rendering infected plants more attractive to specific vectors transmitting these pathogens than uninfected plants, thus potentially aiding in pathogen propagation. Mimicking these chemical cues might enable insect attraction away from the plant or disruption of host finding behavior of the vector. However, the practical implications have not been fully explored. We used citrus, Diaphorina citri and huanglongbing (HLB) as a model host-vector-disease system because HLB threatens citrus production worldwide and is similar to other critical diseases of food crops, such as Zebra Chip affecting potato. We formulated a synthetic chemical blend using selected HLB-specific biomarker compounds, and tested the blend with the Attenu assay system for chemosensory proteins. The Attenu assay system is a procedure that identifies interactions between insect chemosensory proteins and their ligands. We found that mixtures of compounds mimicking the volatile profile of HLB-infected citrus can be bound by chemosensory proteins. Further investigation of these blends in laboratory behavioral assays resulted in development of a synthetic lure that was more attractive to D. citri than natural citrus tree volatiles. This strategy could provide a new route to produce chemical lures for vector population control for a variety of plant and/or animal systems and it may result in the development of a practical lure for monitoring vectors of disease, such as D. citri.

Original languageEnglish (US)
Article number78
JournalFrontiers in Ecology and Evolution
Volume2
Issue numberDEC
DOIs
StatePublished - Dec 9 2014

Fingerprint

blended foods
odor compounds
plant pathogens
greening disease
Diaphorina citri
Citrus
volatile organic compounds
volatile organic compound
disease vectors
pathogen
assay
insect
protein
pathogens
assays
disease vector
insect proteins
host location
host seeking
insects

Keywords

  • Asian citrus psyllid
  • Chemoattractant
  • Citrus greening
  • Lure
  • Odorants protein binding assays
  • VOC

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Synthetic blends of volatile, phytopathogen-induced odorants can be used to manipulate vector behavior. / Aksenov, Alexander A.; Martini, Xavier; Zhao, Weixiang; Stelinski, Lukasz L.; Davis, Cristina E.

In: Frontiers in Ecology and Evolution, Vol. 2, No. DEC, 78, 09.12.2014.

Research output: Contribution to journalArticle

Aksenov, Alexander A. ; Martini, Xavier ; Zhao, Weixiang ; Stelinski, Lukasz L. ; Davis, Cristina E. / Synthetic blends of volatile, phytopathogen-induced odorants can be used to manipulate vector behavior. In: Frontiers in Ecology and Evolution. 2014 ; Vol. 2, No. DEC.
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