Synthetic antigens representing the antigenic variation of human hepatitis C virus

Kyung Hee Kang, Yasuhiro Yamamura, Maria P. Carlos, Nicolas Karvelas, In Sup Kim, Deepa Sunkara, Rebecca Rivera, Murray B. Gardner, David E. Anderson, Francisco Diaz-Mitoma, Jose V Torres, Juan P. Marquez

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Immune responses against hepatitis C virus (HCV) have been studied by numerous groups. However, details concerning the production of antibodies to antigenically variable epitopes remain to be elucidated. Since the sequences of the variable regions of several HCV proteins are different among the virus strains infecting patients, we decided to design peptide combinations that represent the theoretical maximum antigenic variation of each epitope to be used as capture antigens. We prepared six peptide mixtures (hypervariable epitope constructs; HECs) representing six different epitopes from structural and non-structural proteins of HCV from genotypes 1-6. Plasma from 300 HCV patients was tested to determine if their antibodies recognize the synthetic constructs. All the patients were chronically infected with diverse HCV genotypes and did not receive antiviral treatment. Antibodies to one or more of the HECs were detected in all of the HCV-infected individuals. Immunogenicity of the HCV HECs was also evaluated in outbred and inbred mice. Strong HEC-specific antibodies were produced, and cellular responses were also induced that were Th-1 rather than Th-2. Our results show that HCV HECs are both antigens that can be used to detect the broad cross-reactivity of antibodies from HCV-infected patients, and strong immunogens that can induce antigen-specific humoral and cellular immune responses in mice.

Original languageEnglish (US)
Pages (from-to)497-508
Number of pages12
JournalViral Immunology
Volume23
Issue number5
DOIs
StatePublished - Oct 1 2010

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Molecular Medicine

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  • Cite this

    Kang, K. H., Yamamura, Y., Carlos, M. P., Karvelas, N., Kim, I. S., Sunkara, D., Rivera, R., Gardner, M. B., Anderson, D. E., Diaz-Mitoma, F., Torres, J. V., & Marquez, J. P. (2010). Synthetic antigens representing the antigenic variation of human hepatitis C virus. Viral Immunology, 23(5), 497-508. https://doi.org/10.1089/vim.2010.0043