Synthesis of two fluorescent GTPγS molecules and their biological relevance

Denise J. Trans, Ruoli Bai, J. Bennet Addison, Ruiwu Liu, Ernest Hamel, Matthew A Coleman, Paul Henderson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fluorescent GTP analogues are utilized for an assortment of nucleic acid and protein characterization studies. Non-hydrolysable analogues such as GTPγS offer the advantage of keeping proteins in a GTP-bound conformation due to their resistance to hydrolysis into GDP. Two novel fluorescent GTPγS molecules were developed by linking fluorescein and tetramethylrhodamine to the γ-thiophosphate of GTPγS. Chemical and biological analysis of these two compounds revealed their successful synthesis and ability to bind to the nucleotide-binding site of tubulin. These two new fluorescent non-hydrolysable nucleotides offer new possibilities for biophysical and biochemical characterization of GTP-binding proteins.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalNucleosides, Nucleotides and Nucleic Acids
DOIs
StateAccepted/In press - Mar 6 2017

Fingerprint

Guanosine Triphosphate
Nucleotides
Molecules
Tubulin
Fluorescein
GTP-Binding Proteins
Nucleic Acids
Conformations
Hydrolysis
Proteins
Binding Sites
tetramethylrhodamine
thiophosphoric acid

Keywords

  • fluorescent nucleotide analogs
  • FRET
  • NMR and tubulin polymerization
  • Nucleoside and nucleotide analogs

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

Cite this

Synthesis of two fluorescent GTPγS molecules and their biological relevance. / Trans, Denise J.; Bai, Ruoli; Addison, J. Bennet; Liu, Ruiwu; Hamel, Ernest; Coleman, Matthew A; Henderson, Paul.

In: Nucleosides, Nucleotides and Nucleic Acids, 06.03.2017, p. 1-13.

Research output: Contribution to journalArticle

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