Synthesis of mono- and dinucleotide photoaffinity probes of ribonucleic acid polymerase

Leslie H. DeRiemer, Claude F. Meares

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The abortive initiation reaction of RNA polymerase has been used to prepare adenylyl-(3′-5′)-uridine 5′-phosphate (pApU) in 74% yield from AMP and UTP. The reactive intermediate p-azidophenyl phosphorimidazolidate has been prepared by starting from p-nitrophenyl phosphate. Reaction of this compound with the terminal phosphates of adenosine 5′-phosphate and adenylyl-(3′-5′)-uridine 5′-phosphate gives the corresponding β-substituted 5′-diphosphates. These products are incorporated into the 5′ (leading) end of RNA by RNA polymerase (Escherichia coli) and can be photoactivated at a specific stage of RNA elongation. The dinucleotide photoaffinity label β-(4-azidophenyl) adenylyl-(3′-5′)-uridine 5′-diphosphate stimulates RNA synthesis more strongly than adenylyl-(3′-5′)-uridine.

Original languageEnglish (US)
Pages (from-to)1606-1612
Number of pages7
JournalBiochemistry
Volume20
Issue number6
StatePublished - 1981

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Synthesis of mono- and dinucleotide photoaffinity probes of ribonucleic acid polymerase'. Together they form a unique fingerprint.

  • Cite this