Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase

Aimin Song, Xiaobing Wang, Jinhua Zhang, Jan Mařík, Carlito B Lebrilla, Kit Lam

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Four N-Fmoc protected polyoxyethylene-based amino acid type linkers were designed and synthesized for peptide derivatization in solid phase. Three of them were obtained in a crystalline form. The crystallized linkers can be stored at 4°C for 2 years without significant decomposition. Protocols for biotinylation and fluorescent labeling of peptides in solid phase were developed. The linkers also provide good ionization ability for single-bead mass spectrometry analysis of peptides.

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number1
DOIs
StatePublished - Jan 5 2004

Fingerprint

Peptides
Biotinylation
Labeling
Ionization
Mass spectrometry
Mass Spectrometry
Crystalline materials
Decomposition
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase. / Song, Aimin; Wang, Xiaobing; Zhang, Jinhua; Mařík, Jan; Lebrilla, Carlito B; Lam, Kit.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 14, No. 1, 05.01.2004, p. 161-165.

Research output: Contribution to journalArticle

Song, Aimin ; Wang, Xiaobing ; Zhang, Jinhua ; Mařík, Jan ; Lebrilla, Carlito B ; Lam, Kit. / Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase. In: Bioorganic and Medicinal Chemistry Letters. 2004 ; Vol. 14, No. 1. pp. 161-165.
@article{77d8dfab1ca64ebb9701e74ac6b2a19a,
title = "Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase",
abstract = "Four N-Fmoc protected polyoxyethylene-based amino acid type linkers were designed and synthesized for peptide derivatization in solid phase. Three of them were obtained in a crystalline form. The crystallized linkers can be stored at 4°C for 2 years without significant decomposition. Protocols for biotinylation and fluorescent labeling of peptides in solid phase were developed. The linkers also provide good ionization ability for single-bead mass spectrometry analysis of peptides.",
author = "Aimin Song and Xiaobing Wang and Jinhua Zhang and Jan Mař{\'i}k and Lebrilla, {Carlito B} and Kit Lam",
year = "2004",
month = "1",
day = "5",
doi = "10.1016/j.bmcl.2003.09.067",
language = "English (US)",
volume = "14",
pages = "161--165",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase

AU - Song, Aimin

AU - Wang, Xiaobing

AU - Zhang, Jinhua

AU - Mařík, Jan

AU - Lebrilla, Carlito B

AU - Lam, Kit

PY - 2004/1/5

Y1 - 2004/1/5

N2 - Four N-Fmoc protected polyoxyethylene-based amino acid type linkers were designed and synthesized for peptide derivatization in solid phase. Three of them were obtained in a crystalline form. The crystallized linkers can be stored at 4°C for 2 years without significant decomposition. Protocols for biotinylation and fluorescent labeling of peptides in solid phase were developed. The linkers also provide good ionization ability for single-bead mass spectrometry analysis of peptides.

AB - Four N-Fmoc protected polyoxyethylene-based amino acid type linkers were designed and synthesized for peptide derivatization in solid phase. Three of them were obtained in a crystalline form. The crystallized linkers can be stored at 4°C for 2 years without significant decomposition. Protocols for biotinylation and fluorescent labeling of peptides in solid phase were developed. The linkers also provide good ionization ability for single-bead mass spectrometry analysis of peptides.

UR - http://www.scopus.com/inward/record.url?scp=0347517813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347517813&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2003.09.067

DO - 10.1016/j.bmcl.2003.09.067

M3 - Article

C2 - 14684320

AN - SCOPUS:0347517813

VL - 14

SP - 161

EP - 165

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 1

ER -