Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron

Alexander E. Karu, Marvin H. Goodrow, Douglas J. Schmidt, Bruce D. Hammock, Michael W. Bigelow

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Diuron and related phenylurea herbicides and their metabolites are important candidates for sensitive and specific immunodetection. This paper describes a scheme for the synthesis of two different types of phenylurea haptens for immunization and use as detecting conjugates in enzyme immunoassays (EIAs). The haptens were used to develop indirect and direct EIAs and to derive a panel of monoclonal antibodies (MAbs) with different specificities for diuron and its analogs. One of six possible combinations of hapten-spacer arm conjugates tested as immunizing and screening antigens resulted in an indirect competition EIA that was 100-2000-fold more sensitive than the others. The eight most sensitive MAbs had I50 values of 2-20 ppb for diuron. These MAbs gave two different patterns of cross-reactivities with monuron and linuron and negligible recognition of other arylurea herbicides. These MAbs and EIAs are potentially suitable for identification as well as detection of diuron, monuron, and linuron.

Original languageEnglish (US)
Pages (from-to)301-309
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume42
Issue number2
StatePublished - 1994

Fingerprint

phenylurea herbicides
Diuron
haptens
diuron
Haptens
enzyme immunoassays
Herbicides
immunoassays
Immunoenzyme Techniques
Immunoassay
monoclonal antibodies
Linuron
monuron
Monoclonal Antibodies
linuron
synthesis
Enzymes
Immunization
Metabolites
cross reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Karu, A. E., Goodrow, M. H., Schmidt, D. J., Hammock, B. D., & Bigelow, M. W. (1994). Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron. Journal of Agricultural and Food Chemistry, 42(2), 301-309.

Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron. / Karu, Alexander E.; Goodrow, Marvin H.; Schmidt, Douglas J.; Hammock, Bruce D.; Bigelow, Michael W.

In: Journal of Agricultural and Food Chemistry, Vol. 42, No. 2, 1994, p. 301-309.

Research output: Contribution to journalArticle

Karu, AE, Goodrow, MH, Schmidt, DJ, Hammock, BD & Bigelow, MW 1994, 'Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron', Journal of Agricultural and Food Chemistry, vol. 42, no. 2, pp. 301-309.
Karu, Alexander E. ; Goodrow, Marvin H. ; Schmidt, Douglas J. ; Hammock, Bruce D. ; Bigelow, Michael W. / Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron. In: Journal of Agricultural and Food Chemistry. 1994 ; Vol. 42, No. 2. pp. 301-309.
@article{ddd6be5e1f3545fd889e0cfb9b078035,
title = "Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron",
abstract = "Diuron and related phenylurea herbicides and their metabolites are important candidates for sensitive and specific immunodetection. This paper describes a scheme for the synthesis of two different types of phenylurea haptens for immunization and use as detecting conjugates in enzyme immunoassays (EIAs). The haptens were used to develop indirect and direct EIAs and to derive a panel of monoclonal antibodies (MAbs) with different specificities for diuron and its analogs. One of six possible combinations of hapten-spacer arm conjugates tested as immunizing and screening antigens resulted in an indirect competition EIA that was 100-2000-fold more sensitive than the others. The eight most sensitive MAbs had I50 values of 2-20 ppb for diuron. These MAbs gave two different patterns of cross-reactivities with monuron and linuron and negligible recognition of other arylurea herbicides. These MAbs and EIAs are potentially suitable for identification as well as detection of diuron, monuron, and linuron.",
author = "Karu, {Alexander E.} and Goodrow, {Marvin H.} and Schmidt, {Douglas J.} and Hammock, {Bruce D.} and Bigelow, {Michael W.}",
year = "1994",
language = "English (US)",
volume = "42",
pages = "301--309",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "2",

}

TY - JOUR

T1 - Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron

AU - Karu, Alexander E.

AU - Goodrow, Marvin H.

AU - Schmidt, Douglas J.

AU - Hammock, Bruce D.

AU - Bigelow, Michael W.

PY - 1994

Y1 - 1994

N2 - Diuron and related phenylurea herbicides and their metabolites are important candidates for sensitive and specific immunodetection. This paper describes a scheme for the synthesis of two different types of phenylurea haptens for immunization and use as detecting conjugates in enzyme immunoassays (EIAs). The haptens were used to develop indirect and direct EIAs and to derive a panel of monoclonal antibodies (MAbs) with different specificities for diuron and its analogs. One of six possible combinations of hapten-spacer arm conjugates tested as immunizing and screening antigens resulted in an indirect competition EIA that was 100-2000-fold more sensitive than the others. The eight most sensitive MAbs had I50 values of 2-20 ppb for diuron. These MAbs gave two different patterns of cross-reactivities with monuron and linuron and negligible recognition of other arylurea herbicides. These MAbs and EIAs are potentially suitable for identification as well as detection of diuron, monuron, and linuron.

AB - Diuron and related phenylurea herbicides and their metabolites are important candidates for sensitive and specific immunodetection. This paper describes a scheme for the synthesis of two different types of phenylurea haptens for immunization and use as detecting conjugates in enzyme immunoassays (EIAs). The haptens were used to develop indirect and direct EIAs and to derive a panel of monoclonal antibodies (MAbs) with different specificities for diuron and its analogs. One of six possible combinations of hapten-spacer arm conjugates tested as immunizing and screening antigens resulted in an indirect competition EIA that was 100-2000-fold more sensitive than the others. The eight most sensitive MAbs had I50 values of 2-20 ppb for diuron. These MAbs gave two different patterns of cross-reactivities with monuron and linuron and negligible recognition of other arylurea herbicides. These MAbs and EIAs are potentially suitable for identification as well as detection of diuron, monuron, and linuron.

UR - http://www.scopus.com/inward/record.url?scp=0000323126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000323126&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0000323126

VL - 42

SP - 301

EP - 309

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 2

ER -