Abstract
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for quantitative detection of bromacil. Two haptens were synthesized, the handles having been attached at the N-1 and 6-methyl groups of the target molecule; both carboxylic acids were coupled to several proteins to obtain two sets of conjugates and then polyclonal antibodies. The standard curve based on the heterologous system with the antibody raised against immunizing antigen of the latter hapten conjugated to KLH and the coating antigen prepared from the former hapten and BSA was nearly linear at around the 0.25 ppb level, which is promising for the environmental monitoring of bromacil. There was only moderate handle recognition observed. Cross-reactivities for terbacil, a related herbicide, for metabolites of bromacil, and for the major metabolite of terbacil were less than 4%.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1459-1465 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 40 |
| Issue number | 8 |
| State | Published - 1992 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)
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Synthesis of haptens and conjugates for an enzyme immunoassay for analysis of the herbicide bromacilt. / Szurdoki, Ferenc; Bekheit, Hassan K M; Marco, Maria Pilar; Goodrow, Marvin H.; Hammock, Bruce D.
In: Journal of Agricultural and Food Chemistry, Vol. 40, No. 8, 1992, p. 1459-1465.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis of haptens and conjugates for an enzyme immunoassay for analysis of the herbicide bromacilt
AU - Szurdoki, Ferenc
AU - Bekheit, Hassan K M
AU - Marco, Maria Pilar
AU - Goodrow, Marvin H.
AU - Hammock, Bruce D.
PY - 1992
Y1 - 1992
N2 - A competitive enzyme-linked immunosorbent assay (ELISA) was developed for quantitative detection of bromacil. Two haptens were synthesized, the handles having been attached at the N-1 and 6-methyl groups of the target molecule; both carboxylic acids were coupled to several proteins to obtain two sets of conjugates and then polyclonal antibodies. The standard curve based on the heterologous system with the antibody raised against immunizing antigen of the latter hapten conjugated to KLH and the coating antigen prepared from the former hapten and BSA was nearly linear at around the 0.25 ppb level, which is promising for the environmental monitoring of bromacil. There was only moderate handle recognition observed. Cross-reactivities for terbacil, a related herbicide, for metabolites of bromacil, and for the major metabolite of terbacil were less than 4%.
AB - A competitive enzyme-linked immunosorbent assay (ELISA) was developed for quantitative detection of bromacil. Two haptens were synthesized, the handles having been attached at the N-1 and 6-methyl groups of the target molecule; both carboxylic acids were coupled to several proteins to obtain two sets of conjugates and then polyclonal antibodies. The standard curve based on the heterologous system with the antibody raised against immunizing antigen of the latter hapten conjugated to KLH and the coating antigen prepared from the former hapten and BSA was nearly linear at around the 0.25 ppb level, which is promising for the environmental monitoring of bromacil. There was only moderate handle recognition observed. Cross-reactivities for terbacil, a related herbicide, for metabolites of bromacil, and for the major metabolite of terbacil were less than 4%.
UR - http://www.scopus.com/inward/record.url?scp=0007642442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0007642442&partnerID=8YFLogxK
M3 - Article
VL - 40
SP - 1459
EP - 1465
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 8
ER -