Synthesis of haptens and conjugates for an enzyme immunoassay for analysis of the herbicide bromacilt

Ferenc Szurdoki, Hassan K M Bekheit, Maria Pilar Marco, Marvin H. Goodrow, Bruce D. Hammock

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Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) was developed for quantitative detection of bromacil. Two haptens were synthesized, the handles having been attached at the N-1 and 6-methyl groups of the target molecule; both carboxylic acids were coupled to several proteins to obtain two sets of conjugates and then polyclonal antibodies. The standard curve based on the heterologous system with the antibody raised against immunizing antigen of the latter hapten conjugated to KLH and the coating antigen prepared from the former hapten and BSA was nearly linear at around the 0.25 ppb level, which is promising for the environmental monitoring of bromacil. There was only moderate handle recognition observed. Cross-reactivities for terbacil, a related herbicide, for metabolites of bromacil, and for the major metabolite of terbacil were less than 4%.

Original languageEnglish (US)
Pages (from-to)1459-1465
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume40
Issue number8
StatePublished - 1992

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Szurdoki, F., Bekheit, H. K. M., Marco, M. P., Goodrow, M. H., & Hammock, B. D. (1992). Synthesis of haptens and conjugates for an enzyme immunoassay for analysis of the herbicide bromacilt. Journal of Agricultural and Food Chemistry, 40(8), 1459-1465.