Abstract
The experimental antitumor drug Edatrexate, labeled with 99% 13C at the 2-position of the glutamate acid group was required for 13C-magnetic resonance spectroscopy studies in biological media. Coupling of 2,4-diamino-4-deoxy-10-ethyl-10-deazapteroic acid with diethyl L-2-13C-glutamate as promoted by BOP reagent afforded Edatrexate (2-13C-glu) diethyl ester in 60% yield following purification by column chromatography. Saponification by aqueous NaOH in 2-methoxyethanol gave the target molecule in 44% yield or 26% overall.
Original language | English (US) |
---|---|
Pages (from-to) | 99-103 |
Number of pages | 5 |
Journal | Journal of Labelled Compounds and Radiopharmaceuticals |
Volume | 39 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1997 |
Keywords
- C-magnetic resonance spectroscopy
- Antitumor
- Edatrexate
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Medicine
- Analytical Chemistry
- Organic Chemistry
- Drug Discovery
- Pharmacology